Virus Expression Database
Submitted by Justine Smith (Flinders University, Australia) on Jul 05 2017
Platform: ngs – Illumina NextSeq 500 (Homo sapiens)
Pubmed: 28721309
Summary We inoculated ARPE-19 human retinal pigment epithelial cells with EBOV, and followed course of infection by immunocytochemistry and measurement of titer in culture supernatant. To interrogate transcriptional responses of infected cells, we combined RNA sequencing with in silico pathway, gene ontology, transcription factor binding site and network analyses. Human retinal pigment epithelial cells were permissive to infection with EBOV, and supported viral replication and release of virus in high titer. Unexpectedly, 28% of 560 up-regulated transcripts in EBOV-infected cells were type I IFN responsive, indicating a robust type I IFN response.
| ID | Title | Cell Type | Timepoint | Reported Virus | Virus Species | Exclusion Reason |
|---|---|---|---|---|---|---|
| GSM2694827 | 1_24C | retinal pigment epithelium cell line  [ARPE-19  ] |
24 hrs | mock | Uninfected | |
| GSM2694828 | 2_24C | retinal pigment epithelium cell line [ARPE-19 ] |
24 hrs | mock | Uninfected | |
| GSM2694829 | 3_24C | retinal pigment epithelium cell line [ARPE-19 ] |
24 hrs | mock | Uninfected | |
| GSM2694830 | 1_24E | retinal pigment epithelium cell line [ARPE-19 ] |
24 hrs | EBOV | Zaire ebolavirus | |
| GSM2694831 | 2_24E | retinal pigment epithelium cell line [ARPE-19 ] |
24 hrs | EBOV | Zaire ebolavirus | |
| GSM2694832 | 3_24E | retinal pigment epithelium cell line [ARPE-19 ] |
24 hrs | EBOV | Zaire ebolavirus |