Submitted by jinmiao chen (Singapore Immunology Network, Singapore) on Oct 27 2017
Platform: microarray – [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]
Pubmed: 29904383
Summary Transcriptome analysis of mock or H1N1 IAV PR8 infected p53WT A549 and p53null A549-KO3 cells by Affymetrix GeneChip Human Transcriptome 2.0 Arrays to achieve a set of genes those are regulated by p53 and responsive to IAV infection.
Influenza A virus infection activates cellular p53, however it has not been clear whether this process has pro- or anti- viral effects. In this study, using human isogenic p53 wildtype A549 cells and p53null A549-KO3 cells generated from the CRISPR/Cas9 technology, we report that p53null cells exhibit significantly reduced viral propagation property when infected with influenza A virus (H1N1/A/Puerto Rico/8/34). Here, using genome-wide microarray analysis we revealed that p53 regulates the expression of a large set of interferon-inducible genes, some of which are directly associated with viral infectivity and later experimentally validated to be responsible for p53-regulated IAV infectivity.
ID | Title | Cell Type | Timepoint | Reported Virus | Virus Species | Exclusion Reason |
---|---|---|---|---|---|---|
GSM2834531 | A549_Mock A | non-small cell lung adenocarcinoma cell line [A-549 ] | 24 hrs | Mock | Uninfected | |
GSM2834532 | A549_Mock B | non-small cell lung adenocarcinoma cell line [A-549 ] | 24 hrs | Mock | Uninfected | |
GSM2834533 | A549_Mock C | non-small cell lung adenocarcinoma cell line [A-549 ] | 24 hrs | Mock | Uninfected | |
GSM2834534 | A549_Virus A | non-small cell lung adenocarcinoma cell line [A-549 ] | 24 hrs | H1N1 | Influenza A virus | |
GSM2834535 | A549_Virus B | non-small cell lung adenocarcinoma cell line [A-549 ] | 24 hrs | H1N1 | Influenza A virus | |
GSM2834536 | A549_Virus C | non-small cell lung adenocarcinoma cell line [A-549 ] | 24 hrs | H1N1 | Influenza A virus | |
GSM2834537 | A549-KO3_Mock A | non-small cell lung adenocarcinoma cell line [A-549 ] | 24 hrs | Mock | Uninfected | Extra interventions p53 KO cells |
GSM2834538 | A549-KO3_Mock B | non-small cell lung adenocarcinoma cell line [A-549 ] | 24 hrs | Mock | Uninfected | Extra interventions p53 KO cells |
GSM2834539 | A549-KO3_Mock C | non-small cell lung adenocarcinoma cell line [A-549 ] | 24 hrs | Mock | Uninfected | Extra interventions p53 KO cells |
GSM2834540 | A549-KO3_Virus A | non-small cell lung adenocarcinoma cell line [A-549 ] | 24 hrs | H1N1 | Influenza A virus | Extra interventions p53 KO cells |
GSM2834541 | A549-KO3_Virus B | non-small cell lung adenocarcinoma cell line [A-549 ] | 24 hrs | H1N1 | Influenza A virus | Extra interventions p53 KO cells |
GSM2834542 | A549-KO3_Virus C | non-small cell lung adenocarcinoma cell line [A-549 ] | 24 hrs | H1N1 | Influenza A virus | Extra interventions p53 KO cells |