GSE106279

Influenza virus facilitates its infectivity by activating p53 to inhibit the expression of interferon-induced transmembrane proteins (IFITMs)

Submitted by jinmiao chen (Singapore Immunology Network, Singapore) on Oct 27 2017

Platform: microarray – [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]

Pubmed: 29904383

Summary Transcriptome analysis of mock or H1N1 IAV PR8 infected p53WT A549 and p53null A549-KO3 cells by Affymetrix GeneChip Human Transcriptome 2.0 Arrays to achieve a set of genes those are regulated by p53 and responsive to IAV infection.
Influenza A virus infection activates cellular p53, however it has not been clear whether this process has pro- or anti- viral effects. In this study, using human isogenic p53 wildtype A549 cells and p53null A549-KO3 cells generated from the CRISPR/Cas9 technology, we report that p53null cells exhibit significantly reduced viral propagation property when infected with influenza A virus (H1N1/A/Puerto Rico/8/34). Here, using genome-wide microarray analysis we revealed that p53 regulates the expression of a large set of interferon-inducible genes, some of which are directly associated with viral infectivity and later experimentally validated to be responsible for p53-regulated IAV infectivity.

12 Samples

ID	Title	Cell Type	Timepoint	Reported Virus	Virus Species	Exclusion Reason
GSM2834531	A549_Mock A	non-small cell lung adenocarcinoma cell line  [A-549 ]	24 hrs	Mock	Uninfected
GSM2834532	A549_Mock B	non-small cell lung adenocarcinoma cell line  [A-549 ]	24 hrs	Mock	Uninfected
GSM2834533	A549_Mock C	non-small cell lung adenocarcinoma cell line  [A-549 ]	24 hrs	Mock	Uninfected
GSM2834534	A549_Virus A	non-small cell lung adenocarcinoma cell line  [A-549 ]	24 hrs	H1N1	Influenza A virus
GSM2834535	A549_Virus B	non-small cell lung adenocarcinoma cell line  [A-549 ]	24 hrs	H1N1	Influenza A virus
GSM2834536	A549_Virus C	non-small cell lung adenocarcinoma cell line  [A-549 ]	24 hrs	H1N1	Influenza A virus
GSM2834537	A549-KO3_Mock A	non-small cell lung adenocarcinoma cell line  [A-549 ]	24 hrs	Mock	Uninfected	Extra interventions p53 KO cells
GSM2834538	A549-KO3_Mock B	non-small cell lung adenocarcinoma cell line  [A-549 ]	24 hrs	Mock	Uninfected	Extra interventions p53 KO cells
GSM2834539	A549-KO3_Mock C	non-small cell lung adenocarcinoma cell line  [A-549 ]	24 hrs	Mock	Uninfected	Extra interventions p53 KO cells
GSM2834540	A549-KO3_Virus A	non-small cell lung adenocarcinoma cell line  [A-549 ]	24 hrs	H1N1	Influenza A virus	Extra interventions p53 KO cells
GSM2834541	A549-KO3_Virus B	non-small cell lung adenocarcinoma cell line  [A-549 ]	24 hrs	H1N1	Influenza A virus	Extra interventions p53 KO cells
GSM2834542	A549-KO3_Virus C	non-small cell lung adenocarcinoma cell line  [A-549 ]	24 hrs	H1N1	Influenza A virus	Extra interventions p53 KO cells