Virus Expression Database
Submitted by Nicolas Manel (Institut Curie, France) on Jan 24 2018
Platform: microarray – [HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array [transcript (gene) version]
Pubmed: 30270045
Summary Detection of viruses by innate immune sensors induces protective antiviral immunity. The viral DNA sensor cGAS is necessary for detection of HIV by human dendritic cells and macrophages. However, synthesis of HIV DNA during infection is not sufficient for immune activation. The capsid protein, which associates with viral DNA, has a pivotal role in enabling cGAS-mediated immune activation. We now find that NONO is an essential sensor of the HIV capsid in the nucleus. NONO protein directly binds capsid with higher affinity for weakly pathogenic HIV-2 than highly pathogenic HIV-1. Upon infection, NONO is essential for cGAS activation by HIV and cGAS association with HIV DNA in the nucleus. NONO recognizes a conserved region in HIV capsid with limited tolerance for escape mutations. Detection of nuclear viral capsid by NONO to promote DNA sensing by cGAS reveals an innate strategy to achieve distinction of viruses from self in the nucleus.
| ID | Title | Cell Type | Timepoint | Reported Virus | Virus Species | Exclusion Reason |
|---|---|---|---|---|---|---|
| GSM2946172 | MDDC-NI-rep1 | monocyte-derived dendritic cell  |
24 hrs | non-infected | Uninfected | Data failed QCall_probeset_mad_residual_mean is 0.846037 (poor normalization, above threshold of 0.80) |
| GSM2946173 | MDDC-HIV1-Vpx-rep1 | monocyte-derived dendritic cell |
24 hrs | HIV1-Vpx | Human immunodeficiency virus 1 | Data failed QCall_probeset_mad_residual_mean is 0.953471 (poor normalization, above threshold of 0.80) |
| GSM2946174 | MDDC-HIV2-rep1 | monocyte-derived dendritic cell |
24 hrs | HIV2 | Human immunodeficiency virus 2 | Data failed QCall_probeset_mad_residual_mean is 0.807488 (poor normalization, above threshold of 0.80) |
| GSM2946175 | MDDC-NI-rep2 | monocyte-derived dendritic cell |
24 hrs | non-infected | Uninfected | Data failed QCall_probeset_mad_residual_mean is 0.824393 (poor normalization, above threshold of 0.80) |
| GSM2946176 | MDDC-HIV1-Vpx-rep2 | monocyte-derived dendritic cell |
24 hrs | HIV1-Vpx | Human immunodeficiency virus 1 | Data failed QCall_probeset_mad_residual_mean is 0.895416 (poor normalization, above threshold of 0.80) |
| GSM2946177 | MDDC-HIV2-rep2 | monocyte-derived dendritic cell |
24 hrs | HIV2 | Human immunodeficiency virus 2 | Data failed QCall_probeset_mad_residual_mean is 0.888357 (poor normalization, above threshold of 0.80) |
| GSM2946178 | MDDC-NI-rep3 | monocyte-derived dendritic cell |
24 hrs | non-infected | Uninfected | Data failed QCall_probeset_mad_residual_mean is 0.808988 (poor normalization, above threshold of 0.80) |
| GSM2946179 | MDDC-HIV1-Vpx-rep3 | monocyte-derived dendritic cell |
24 hrs | HIV1-Vpx | Human immunodeficiency virus 1 | Data failed QCall_probeset_mad_residual_mean is 0.8718 (poor normalization, above threshold of 0.80) |
| GSM2946180 | MDDC-HIV2-rep3 | monocyte-derived dendritic cell |
24 hrs | HIV2 | Human immunodeficiency virus 2 | Data failed QCall_probeset_mad_residual_mean is 0.819244 (poor normalization, above threshold of 0.80) |
| GSM2946181 | MDDC-NI-rep4 | monocyte-derived dendritic cell |
24 hrs | non-infected | Uninfected | Data failed QCall_probeset_mad_residual_mean is 0.810385 (poor normalization, above threshold of 0.80) |
| GSM2946182 | MDDC-HIV1-Vpx-rep4 | monocyte-derived dendritic cell |
24 hrs | HIV1-Vpx | Human immunodeficiency virus 1 | Data failed QCall_probeset_mad_residual_mean is 0.917146 (poor normalization, above threshold of 0.80) |
| GSM2946183 | MDDC-HIV2-rep4 | monocyte-derived dendritic cell |
24 hrs | HIV2 | Human immunodeficiency virus 2 | Data failed QCall_probeset_mad_residual_mean is 0.960674 (poor normalization, above threshold of 0.80) |
| GSM2946184 | MDDC-cGAMP4-rep1 | monocyte-derived dendritic cell |
24 hrs | cGAMP4 | Uninfected | Extra interventions cGAMP with lipofectamin |
| GSM2946185 | MDDC-NI-rep5 | monocyte-derived dendritic cell |
24 hrs | non-infected | Uninfected | Data failed QCall_probeset_mad_residual_mean is 0.914597 (poor normalization, above threshold of 0.80) |
| GSM2946186 | MDDC-HIV1-Vpx-rep5 | monocyte-derived dendritic cell |
24 hrs | HIV1-Vpx | Human immunodeficiency virus 1 | Data failed QCall_probeset_mad_residual_mean is 1.019141 (poor normalization, above threshold of 0.80) |
| GSM2946187 | MDDC-HIV2-rep5 | monocyte-derived dendritic cell |
24 hrs | HIV2 | Human immunodeficiency virus 2 | Data failed QCall_probeset_mad_residual_mean is 0.976806 (poor normalization, above threshold of 0.80) |
| GSM2946188 | MDDC-cGAMP4-rep2 | monocyte-derived dendritic cell |
24 hrs | cGAMP4 | Uninfected | Extra interventions cGAMP with lipofectamin |
| GSM2946189 | MDDC-NI-rep6 | monocyte-derived dendritic cell |
24 hrs | non-infected | Uninfected | Data failed QCall_probeset_mad_residual_mean is 0.846998 (poor normalization, above threshold of 0.80) |
| GSM2946190 | MDDC-HIV1-Vpx-rep6 | monocyte-derived dendritic cell |
24 hrs | HIV1-Vpx | Human immunodeficiency virus 1 | Data failed QCall_probeset_mad_residual_mean is 0.83101 (poor normalization, above threshold of 0.80) |
| GSM2946191 | MDDC-HIV2-rep6 | monocyte-derived dendritic cell |
24 hrs | HIV2 | Human immunodeficiency virus 2 | Data failed QCall_probeset_mad_residual_mean is 0.901439 (poor normalization, above threshold of 0.80) |
| GSM2946192 | MDDC-cGAMP4-rep3 | monocyte-derived dendritic cell |
24 hrs | cGAMP4 | Uninfected | Extra interventions cGAMP with lipofectamin |