Virus Expression Database

GSE12720

Unique early gene expression patterns in adult to adult living donor liver grafts compared to deceased donor grafts

Submitted by Sunil Kurian (The Scripps Research Institute, USA) on Sep 10 2008

Platform: microarray – [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

Pubmed: None listed

Summary Because of inherent differences between deceased donor (DD) and living donor (LD) liver grafts, we hypothesize that the molecular signatures will be unique, correlating with specific biologic pathways and clinical patterns. Following reperfusion, 579 genes in DD grafts and 1324 genes in LDs were differentially expressed (p<0.005). Many up-regulated LD genes were related to regeneration, biosynthesis and cell cycle, and a large number of down-regulated genes were linked to hepatic metabolism and energy pathways correlating with post-transplant clinical laboratory findings. There was significant up-regulation of inflammatory/immune genes in both DD and LD, each with a distinct pattern. Gene expression patterns of select genes associated with inflammation and regeneration in LD and DD grafts correlated with protein expression.
Unique patterns of early gene expression are seen in LD and DD liver grafts, correlating with protein expression and clinical results, demonstrating distinct inflammatory profiles and significant down-regulation of metabolic pathways in LD grafts.

Keywords: liver transplantation, live donor transplantation, liver regeneration, microarrays, mRNA expression, reperfusion injury

63 Samples

ID Title Cell Type Timepoint Reported Virus Virus Species Exclusion Reason
GSM319257 LivPAPost liver  none Uninfected
GSM319258 LivVCUDon(RC) liver  HCV Hepacivirus C
GSM319259 LivVCUPost(RC) liver  HCV Hepacivirus C
GSM319260 LivVCUPre(RC) liver  HCV Hepacivirus C
GSM319261 Liv19Pre liver  HCV Hepacivirus C
GSM319262 Liv20Don liver  HCV Hepacivirus C Data failed QC
all_probeset_mad_residual_mean is 0.90867 (poor normalization, above threshold of 0.80)
GSM319263 Liv20Post liver  HCV Hepacivirus C
GSM319264 Liv21Pre liver  HCV Hepacivirus C
GSM319265 Liv23Don liver  none Uninfected
GSM319266 Liv23Post liver  none Uninfected
GSM319267 Liv24Don liver  HCV Hepacivirus C
GSM319268 Liv24Pre liver  HCV Hepacivirus C
GSM319269 Liv19 Don liver  HCV Hepacivirus C Data failed QC
all_probeset_mad_residual_mean is 0.863476 (poor normalization, above threshold of 0.80)
GSM319270 Liv19Post liver  HCV Hepacivirus C
GSM319271 Liv20Pre liver  HCV Hepacivirus C
GSM319272 Liv21Don liver  HCV Hepacivirus C Data failed QC
all_probeset_mad_residual_mean is 0.803878 (poor normalization, above threshold of 0.80)
GSM319273 Liv21Post liver  HCV Hepacivirus C
GSM319274 Liv23Pre liver  none Uninfected
GSM319275 Liv24Post liver  HCV Hepacivirus C
GSM319276 Liv25Don liver  HCV Hepacivirus C
GSM319277 Liv26Pre liver  none Uninfected
GSM319278 Liv29Post liver  none Uninfected
GSM319279 Liv31Don liver  HCV Hepacivirus C
GSM319280 Liv32Pre liver  HCV Hepacivirus C
GSM319281 Liv33Post liver  none Uninfected
GSM319282 Liv42Don liver  none Uninfected
GSM319283 Liv46Pre liver  none Uninfected
GSM319284 Liv26Don liver  none Uninfected
GSM319285 Liv29Pre liver  none Uninfected
GSM319286 Liv32Don liver  HCV Hepacivirus C
GSM319287 Liv47Post liver  none Uninfected
GSM319288 Liv48Don liver  none Uninfected
GSM319289 Liv49Pre liver  HCV Hepacivirus C
GSM319290 Liv50Post liver  none Uninfected
GSM319291 Liv25Post liver  HCV Hepacivirus C
GSM319292 Liv33Pre liver  none Uninfected
GSM319293 Liv42Post liver  none Uninfected
GSM319294 Liv46Don liver  none Uninfected
GSM319295 Liv47Pre liver  none Uninfected
GSM319296 Liv48Post liver  none Uninfected
GSM319297 Liv49Don liver  HCV Hepacivirus C
GSM319298 Liv50Pre liver  none Uninfected
GSM319299 Liv29Don liver  none Uninfected
GSM319300 Liv31Pre liver  HCV Hepacivirus C
GSM319301 Liv32Post liver  HCV Hepacivirus C
GSM319302 Liv33Don liver  none Uninfected
GSM319303 Liv42Pre liver  none Uninfected
GSM319304 Liv46Post liver  none Uninfected
GSM319305 Liv47Don liver  none Uninfected
GSM319306 Liv48Pre liver  none Uninfected
GSM319307 Liv25Pre liver  HCV Hepacivirus C
GSM319308 Liv26Post liver  none Uninfected
GSM319309 Liv31Post liver  HCV Hepacivirus C
GSM319310 Liv49Post liver  HCV Hepacivirus C
GSM319311 Liv50Don liver  none Uninfected
GSM319312 Liv27Don liver  none Uninfected
GSM319313 Liv27Pre liver  none Uninfected
GSM319314 Liv30Post liver  HCV Hepacivirus C
GSM319315 LivPAPre liver  none Uninfected
GSM319316 Liv27Post liver  none Uninfected
GSM319317 Liv30Don liver  HCV Hepacivirus C
GSM319318 Liv30Pre liver  HCV Hepacivirus C
GSM319319 LivPADon liver  none Uninfected