GSE131504

Characterization of Human Pegivirus Infection in Liver Transplantation Recipients

Submitted by Daisuke Okuzaki (Research institute for microbial diseases, Japan) on May 20 2019

Platform: ngs – Illumina HiSeq 2500 (Homo sapiens)

Pubmed: 31350911

Summary Approximately 2% of heathy persons are infected with human pegivirus (HPgV). HPgV is transmitted via vertical, sexual, and blood-borne routes. Recently, the association of HPgV infection with the risk of lymphoma was reported. We examined the prevalence of chronic HPgV infection in liver transplantation (LT) recipients and hepatectomy patients and the influence of HPgV infection after LT on clinical and perioperative factors. We enrolled 313 LT recipients and 187 hepatectomy patients who received care at the Kyusyu University Hospital between May 1997 and September 2017. Total RNA was extracted from peripheral blood samples of patients/recipients collected postoperatively. HPgV RNA was measured using real-time polymerase chain reaction (RT-PCR). Of the 313 recipients and 187 patients enrolled in this study, 44 recipients (14.1%) and two patients (1.1%) had HPgV viremia. There was no significant association between HPgV infection and LT outcomes. Interestingly, one recipient was infected with HPgV during the peritransplant period, which was likely transmitted via blood transfusion as HPgV RNA was detected from the blood bag transfused to the recipient during LT. Additionally, HPgV infection induced the upregulation of interferon stimulated gene (ISG) expression in peripheral blood mononuclear cells (PBMCs). LT recipients had higher HPgV viremia compared to hepatectomy patients. Although HPgV infection was not associated with LT-related outcomes, it induced ISG expression in recipients.

6 Samples

ID	Title	Cell Type	Timepoint	Reported Virus	Virus Species
GSM3782942	HPgV-positive PBMCs_rep1	peripheral blood mononuclear cell	chronic	HPgV	Pegivirus C
GSM3782943	HPgV-positive PBMCs_rep2	peripheral blood mononuclear cell	chronic	HPgV	Pegivirus C
GSM3782944	HPgV-positive PBMCs_rep3	peripheral blood mononuclear cell	chronic	HPgV	Pegivirus C
GSM3782945	HPgV-negative PBMCs_rep1	peripheral blood mononuclear cell		none	Uninfected
GSM3782946	HPgV-negative PBMCs_rep2	peripheral blood mononuclear cell		none	Uninfected
GSM3782947	HPgV-negative PBMCs_rep3	peripheral blood mononuclear cell		none	Uninfected