
Submitted by Jaewon Song (Seoul National University, South Korea) on Jun 03 2019
Platform: ngs – Illumina HiSeq 2500 (Homo sapiens) 
Pubmed: 31451648
Summary To investigate how Roquin regulates cellular transcripts during Human cytomegalovirus (HCMV) infection, we examined the levels of cellular transcripts in cells treated control or Roquin-targeting siRNA during HCMV replication. Also, we performed Roquin crosslinking and immunoprecipitation followed by high-throughput sequencing (Roquin CLIP-seq) in HCMV-infected cells to identify which transcripts are directly bound by Roquin.
| ID | Title | Cell Type | Timepoint | Reported Virus | Virus Species | Exclusion Reason |
|---|---|---|---|---|---|---|
| GSM3842213 | KD01: siCon, NI, Rep#1 | foreskin fibroblast cell line ![]() |
none | Uninfected | ||
| GSM3842214 | KD02: siCon, 06h, Rep#1 | foreskin fibroblast cell line ![]() |
6 hrs | HCMV | Human betaherpesvirus 5 | |
| GSM3842215 | KD03: siCon, 24h, Rep#1 | foreskin fibroblast cell line ![]() |
24 hrs | HCMV | Human betaherpesvirus 5 | |
| GSM3842216 | KD04: siCon, 72h, Rep#1 | foreskin fibroblast cell line ![]() |
72 hrs | HCMV | Human betaherpesvirus 5 | |
| GSM3842217 | KD05: siRoq#1, NI, Rep#1 | foreskin fibroblast cell line ![]() |
none | Uninfected | Extra interventions siRNA vs Roquin |
|
| GSM3842218 | KD06: siRoq#1, 06h, Rep#1 | foreskin fibroblast cell line ![]() |
6 hrs | HCMV | Human betaherpesvirus 5 | Extra interventions siRNA vs Roquin |
| GSM3842219 | KD07: siRoq#1, 24h, Rep#1 | foreskin fibroblast cell line ![]() |
24 hrs | HCMV | Human betaherpesvirus 5 | Extra interventions siRNA vs Roquin |
| GSM3842220 | KD08: siRoq#1, 72h, Rep#1 | foreskin fibroblast cell line ![]() |
72 hrs | HCMV | Human betaherpesvirus 5 | Extra interventions siRNA vs Roquin |
| GSM3842221 | KD09: siRoq#2, NI, Rep#1 | foreskin fibroblast cell line ![]() |
none | Uninfected | Extra interventions siRNA vs Roquin |
|
| GSM3842222 | KD10: siRoq#2, 06h, Rep#1 | foreskin fibroblast cell line ![]() |
6 hrs | HCMV | Human betaherpesvirus 5 | Extra interventions siRNA vs Roquin |
| GSM3842223 | KD11: siRoq#2, 24h, Rep#1 | foreskin fibroblast cell line ![]() |
24 hrs | HCMV | Human betaherpesvirus 5 | Extra interventions siRNA vs Roquin |
| GSM3842224 | KD12: siRoq#2, 72h, Rep#1 | foreskin fibroblast cell line ![]() |
72 hrs | HCMV | Human betaherpesvirus 5 | Extra interventions siRNA vs Roquin |
| GSM3842225 | KD13: siCon, NI, Rep#2 | foreskin fibroblast cell line ![]() |
none | Uninfected | ||
| GSM3842226 | KD14: siCon, 06h, Rep#2 | foreskin fibroblast cell line ![]() |
6 hrs | HCMV | Human betaherpesvirus 5 | |
| GSM3842227 | KD15: siCon, 24h, Rep#2 | foreskin fibroblast cell line ![]() |
24 hrs | HCMV | Human betaherpesvirus 5 | |
| GSM3842228 | KD16: siCon, 72h, Rep#2 | foreskin fibroblast cell line ![]() |
72 hrs | HCMV | Human betaherpesvirus 5 | |
| GSM3842229 | KD17: siRoq#1, NI, Rep#2 | foreskin fibroblast cell line ![]() |
none | Uninfected | Extra interventions siRNA vs Roquin |
|
| GSM3842230 | KD18: siRoq#1, 06h, Rep#2 | foreskin fibroblast cell line ![]() |
6 hrs | HCMV | Human betaherpesvirus 5 | Extra interventions siRNA vs Roquin |
| GSM3842231 | KD19: siRoq#1, 24h, Rep#2 | foreskin fibroblast cell line ![]() |
24 hrs | HCMV | Human betaherpesvirus 5 | Extra interventions siRNA vs Roquin |
| GSM3842232 | KD20: siRoq#1, 72h, Rep#2 | foreskin fibroblast cell line ![]() |
72 hrs | HCMV | Human betaherpesvirus 5 | Extra interventions siRNA vs Roquin |
| GSM3842233 | KD21: siRoq#2, NI, Rep#2 | foreskin fibroblast cell line ![]() |
none | Uninfected | Extra interventions siRNA vs Roquin |
|
| GSM3842234 | KD22: siRoq#2, 06h, Rep#2 | foreskin fibroblast cell line ![]() |
6 hrs | HCMV | Human betaherpesvirus 5 | Extra interventions siRNA vs Roquin |
| GSM3842235 | KD23: siRoq#2, 24h, Rep#2 | foreskin fibroblast cell line ![]() |
24 hrs | HCMV | Human betaherpesvirus 5 | Extra interventions siRNA vs Roquin |
| GSM3842236 | KD24: siRoq#2, 72h, Rep#2 | foreskin fibroblast cell line ![]() |
72 hrs | HCMV | Human betaherpesvirus 5 | Extra interventions siRNA vs Roquin |