Submitted by Daniel Todt (Ruhr University Bochum, Germany) on Aug 09 2019
Platform: ngs – Illumina NovaSeq 6000 (Homo sapiens)
Pubmed: 31896581
Summary In this study, we established a protocol based on the HEV genotype 3 p6 (Kernow C-1) and the human hepatoma cell lines HepG2 and HepG2/C3A with different media conditions to produce HEV cell culture-derived particles (HEVcc) with viral titers between 10e5 to 10e6 FFU/mL. Viral titers could be further enhanced by an HEV variant harboring a mutation in the RNA-dependent RNA polymerase. These HEVcc particles were infectious in primary human hepatocytes. RNA sequencing studies of HEV infected primary human hepatocytes demonstrated a temporally structured transcriptional defense response. In conclusion, this robust cell culture model of HEV infection provides a powerful tool for studying viral-host interactions that should facilitate the discovery of antiviral drugs and vaccines for this important zoonotic pathogen.
ID | Title | Cell Type | Timepoint | Reported Virus | Virus Species | Exclusion Reason |
---|---|---|---|---|---|---|
GSM4023557 | 1_4h_MOI_1_untreated | hepatocyte | 4 hrs | HEV | Orthohepevirus A | |
GSM4023558 | 1_8h_MOI_1_untreated | hepatocyte | 8 hrs | HEV | Orthohepevirus A | |
GSM4023559 | 1_12h_MOI_1_untreated | hepatocyte | 12 hrs | HEV | Orthohepevirus A | |
GSM4023560 | 1_24h_MOI_1_untreated | hepatocyte | 24 hrs | HEV | Orthohepevirus A | |
GSM4023561 | 1_48h_MOI_1_untreated | hepatocyte | 48 hrs | HEV | Orthohepevirus A | |
GSM4023562 | 1_7d_MOI_1_untreated | hepatocyte | 7 days | HEV | Orthohepevirus A | |
GSM4023563 | 2_4h_MOI_1_25uM_RBV | hepatocyte | 4 hrs | HEV | Orthohepevirus A | Extra interventions Ribovarin treated |
GSM4023564 | 2_8h_MOI_1_25uM_RBV | hepatocyte | 8 hrs | HEV | Orthohepevirus A | Extra interventions Ribovarin treated |
GSM4023565 | 2_12h_MOI_1_25uM_RBV | hepatocyte | 12 hrs | HEV | Orthohepevirus A | Extra interventions Ribovarin treated |
GSM4023566 | 2_24h_MOI_1_25uM_RBV | hepatocyte | 24 hrs | HEV | Orthohepevirus A | Extra interventions Ribovarin treated |
GSM4023567 | 2_48h_MOI_1_25uM_RBV | hepatocyte | 48 hrs | HEV | Orthohepevirus A | Extra interventions Ribovarin treated |
GSM4023568 | 2_7d_MOI_1_25uM_RBV | hepatocyte | 7 days | HEV | Orthohepevirus A | Extra interventions Ribovarin treated |
GSM4023569 | 3_4h_mock_untreated | hepatocyte | 4 hrs | mock | Uninfected | |
GSM4023570 | 3_8h_mock_untreated | hepatocyte | 8 hrs | mock | Uninfected | |
GSM4023571 | 3_12h_mock_untreated | hepatocyte | 12 hrs | mock | Uninfected | |
GSM4023572 | 3_24h_mock_untreated | hepatocyte | 24 hrs | mock | Uninfected | |
GSM4023573 | 3_48h_mock_untreated | hepatocyte | 48 hrs | mock | Uninfected | |
GSM4023574 | 3_7d_mock_untreated | hepatocyte | 7 days | mock | Uninfected | |
GSM4023575 | 4_4h_mock_1_25uM_RBV | hepatocyte | 4 hrs | mock | Uninfected | Extra interventions Ribovarin treated |
GSM4023576 | 4_8h_mock_1_25uM_RBV | hepatocyte | 8 hrs | mock | Uninfected | Extra interventions Ribovarin treated |
GSM4023577 | 4_12h_mock_1_25uM_RBV | hepatocyte | 12 hrs | mock | Uninfected | Extra interventions Ribovarin treated |
GSM4023578 | 4_24h_mock_1_25uM_RBV | hepatocyte | 24 hrs | mock | Uninfected | Extra interventions Ribovarin treated |
GSM4023579 | 4_48h_mock_1_25uM_RBV | hepatocyte | 48 hrs | mock | Uninfected | Extra interventions Ribovarin treated |
GSM4023580 | 4_7d_mock_1_25uM_RBV | hepatocyte | 7 days | mock | Uninfected | Extra interventions Ribovarin treated |