GSE135619

Robust hepatitis E virus infection and transcriptional response in human hepatocytes

Submitted by Daniel Todt (Ruhr University Bochum, Germany) on Aug 09 2019

Platform: ngs – Illumina NovaSeq 6000 (Homo sapiens)

Pubmed: 31896581

Summary In this study, we established a protocol based on the HEV genotype 3 p6 (Kernow C-1) and the human hepatoma cell lines HepG2 and HepG2/C3A with different media conditions to produce HEV cell culture-derived particles (HEVcc) with viral titers between 10e5 to 10e6 FFU/mL. Viral titers could be further enhanced by an HEV variant harboring a mutation in the RNA-dependent RNA polymerase. These HEVcc particles were infectious in primary human hepatocytes. RNA sequencing studies of HEV infected primary human hepatocytes demonstrated a temporally structured transcriptional defense response. In conclusion, this robust cell culture model of HEV infection provides a powerful tool for studying viral-host interactions that should facilitate the discovery of antiviral drugs and vaccines for this important zoonotic pathogen.

24 Samples

ID	Title	Cell Type	Timepoint	Reported Virus	Virus Species	Exclusion Reason
GSM4023557	1_4h_MOI_1_untreated	hepatocyte	4 hrs	HEV	Orthohepevirus A
GSM4023558	1_8h_MOI_1_untreated	hepatocyte	8 hrs	HEV	Orthohepevirus A
GSM4023559	1_12h_MOI_1_untreated	hepatocyte	12 hrs	HEV	Orthohepevirus A
GSM4023560	1_24h_MOI_1_untreated	hepatocyte	24 hrs	HEV	Orthohepevirus A
GSM4023561	1_48h_MOI_1_untreated	hepatocyte	48 hrs	HEV	Orthohepevirus A
GSM4023562	1_7d_MOI_1_untreated	hepatocyte	7 days	HEV	Orthohepevirus A
GSM4023563	2_4h_MOI_1_25uM_RBV	hepatocyte	4 hrs	HEV	Orthohepevirus A	Extra interventions Ribovarin treated
GSM4023564	2_8h_MOI_1_25uM_RBV	hepatocyte	8 hrs	HEV	Orthohepevirus A	Extra interventions Ribovarin treated
GSM4023565	2_12h_MOI_1_25uM_RBV	hepatocyte	12 hrs	HEV	Orthohepevirus A	Extra interventions Ribovarin treated
GSM4023566	2_24h_MOI_1_25uM_RBV	hepatocyte	24 hrs	HEV	Orthohepevirus A	Extra interventions Ribovarin treated
GSM4023567	2_48h_MOI_1_25uM_RBV	hepatocyte	48 hrs	HEV	Orthohepevirus A	Extra interventions Ribovarin treated
GSM4023568	2_7d_MOI_1_25uM_RBV	hepatocyte	7 days	HEV	Orthohepevirus A	Extra interventions Ribovarin treated
GSM4023569	3_4h_mock_untreated	hepatocyte	4 hrs	mock	Uninfected
GSM4023570	3_8h_mock_untreated	hepatocyte	8 hrs	mock	Uninfected
GSM4023571	3_12h_mock_untreated	hepatocyte	12 hrs	mock	Uninfected
GSM4023572	3_24h_mock_untreated	hepatocyte	24 hrs	mock	Uninfected
GSM4023573	3_48h_mock_untreated	hepatocyte	48 hrs	mock	Uninfected
GSM4023574	3_7d_mock_untreated	hepatocyte	7 days	mock	Uninfected
GSM4023575	4_4h_mock_1_25uM_RBV	hepatocyte	4 hrs	mock	Uninfected	Extra interventions Ribovarin treated
GSM4023576	4_8h_mock_1_25uM_RBV	hepatocyte	8 hrs	mock	Uninfected	Extra interventions Ribovarin treated
GSM4023577	4_12h_mock_1_25uM_RBV	hepatocyte	12 hrs	mock	Uninfected	Extra interventions Ribovarin treated
GSM4023578	4_24h_mock_1_25uM_RBV	hepatocyte	24 hrs	mock	Uninfected	Extra interventions Ribovarin treated
GSM4023579	4_48h_mock_1_25uM_RBV	hepatocyte	48 hrs	mock	Uninfected	Extra interventions Ribovarin treated
GSM4023580	4_7d_mock_1_25uM_RBV	hepatocyte	7 days	mock	Uninfected	Extra interventions Ribovarin treated