Virus Expression Database

GSE135619

Robust hepatitis E virus infection and transcriptional response in human hepatocytes

Submitted by Daniel Todt (Ruhr University Bochum, Germany) on Aug 09 2019

Platform: ngs – Illumina NovaSeq 6000 (Homo sapiens)

Pubmed: 31896581

Summary In this study, we established a protocol based on the HEV genotype 3 p6 (Kernow C-1) and the human hepatoma cell lines HepG2 and HepG2/C3A with different media conditions to produce HEV cell culture-derived particles (HEVcc) with viral titers between 10e5 to 10e6 FFU/mL. Viral titers could be further enhanced by an HEV variant harboring a mutation in the RNA-dependent RNA polymerase. These HEVcc particles were infectious in primary human hepatocytes. RNA sequencing studies of HEV infected primary human hepatocytes demonstrated a temporally structured transcriptional defense response. In conclusion, this robust cell culture model of HEV infection provides a powerful tool for studying viral-host interactions that should facilitate the discovery of antiviral drugs and vaccines for this important zoonotic pathogen.

24 Samples

ID Title Cell Type Timepoint Reported Virus Virus Species Exclusion Reason
GSM4023557 1_4h_MOI_1_untreated hepatocyte  4 hrs HEV Orthohepevirus A
GSM4023558 1_8h_MOI_1_untreated hepatocyte  8 hrs HEV Orthohepevirus A
GSM4023559 1_12h_MOI_1_untreated hepatocyte  12 hrs HEV Orthohepevirus A
GSM4023560 1_24h_MOI_1_untreated hepatocyte  24 hrs HEV Orthohepevirus A
GSM4023561 1_48h_MOI_1_untreated hepatocyte  48 hrs HEV Orthohepevirus A
GSM4023562 1_7d_MOI_1_untreated hepatocyte  7 days HEV Orthohepevirus A
GSM4023563 2_4h_MOI_1_25uM_RBV hepatocyte  4 hrs HEV Orthohepevirus A Extra interventions
Ribovarin treated
GSM4023564 2_8h_MOI_1_25uM_RBV hepatocyte  8 hrs HEV Orthohepevirus A Extra interventions
Ribovarin treated
GSM4023565 2_12h_MOI_1_25uM_RBV hepatocyte  12 hrs HEV Orthohepevirus A Extra interventions
Ribovarin treated
GSM4023566 2_24h_MOI_1_25uM_RBV hepatocyte  24 hrs HEV Orthohepevirus A Extra interventions
Ribovarin treated
GSM4023567 2_48h_MOI_1_25uM_RBV hepatocyte  48 hrs HEV Orthohepevirus A Extra interventions
Ribovarin treated
GSM4023568 2_7d_MOI_1_25uM_RBV hepatocyte  7 days HEV Orthohepevirus A Extra interventions
Ribovarin treated
GSM4023569 3_4h_mock_untreated hepatocyte  4 hrs mock Uninfected
GSM4023570 3_8h_mock_untreated hepatocyte  8 hrs mock Uninfected
GSM4023571 3_12h_mock_untreated hepatocyte  12 hrs mock Uninfected
GSM4023572 3_24h_mock_untreated hepatocyte  24 hrs mock Uninfected
GSM4023573 3_48h_mock_untreated hepatocyte  48 hrs mock Uninfected
GSM4023574 3_7d_mock_untreated hepatocyte  7 days mock Uninfected
GSM4023575 4_4h_mock_1_25uM_RBV hepatocyte  4 hrs mock Uninfected Extra interventions
Ribovarin treated
GSM4023576 4_8h_mock_1_25uM_RBV hepatocyte  8 hrs mock Uninfected Extra interventions
Ribovarin treated
GSM4023577 4_12h_mock_1_25uM_RBV hepatocyte  12 hrs mock Uninfected Extra interventions
Ribovarin treated
GSM4023578 4_24h_mock_1_25uM_RBV hepatocyte  24 hrs mock Uninfected Extra interventions
Ribovarin treated
GSM4023579 4_48h_mock_1_25uM_RBV hepatocyte  48 hrs mock Uninfected Extra interventions
Ribovarin treated
GSM4023580 4_7d_mock_1_25uM_RBV hepatocyte  7 days mock Uninfected Extra interventions
Ribovarin treated