Virus Expression Database
Submitted by Shuibing Chen (Weill Cornell Medical College, USA) on Jun 05 2020
Platform: ngs – Illumina NextSeq 500 (Homo sapiens)
Pubmed: 33236003
Summary Heart injury has been reported in up to 20% of COVID-19 patients, yet the cause of myocardial histopathology is unknown. We examined heart tissue from autopsies of healthy and COVID-19 patients using RNA-seq and identified a strikingly increased CCL2 expression and macrophage population in COVID-19 heart sample. Transwell assays using hESC-derived cardiomyocytes (CMs) and macrophages revealed that SARS-CoV-2 infected CMs secrete CCL2, which recruits macrophages, and this was validated using adult human CMs. Macrophages recruited by CCL2 from infected CMs secrete IL-6 and TNF-α, which causes increased ROS and cell apoptosis of CMs. Finally, a high content chemical screen using FDA-approved drugs identified ranolozine and tofacitinib, which rescue SARS-CoV-2 infected CMs from macrophages-induced cardiotoxicity.
| ID | Title | Cell Type | Timepoint | Reported Virus | Virus Species | Exclusion Reason |
|---|---|---|---|---|---|---|
| GSM4592626 | hESC-derived cardiomyocytes_Mock_1 | cardiomyocyte cell line  |
Mock | Uninfected | ||
| GSM4592627 | hESC-derived cardiomyocytes_Mock_2 | cardiomyocyte cell line |
Mock | Uninfected | ||
| GSM4592628 | hESC-derived cardiomyocytes_Mock_3 | cardiomyocyte cell line |
Mock | Uninfected | ||
| GSM4592629 | hESC-derived cardiomyocytes_SARS-CoV-2_1 | cardiomyocyte cell line |
SARS-CoV-2 | Severe acute respiratory syndrome-related coronavirus | ||
| GSM4592630 | hESC-derived cardiomyocytes_SARS-CoV-2_2 | cardiomyocyte cell line |
SARS-CoV-2 | Severe acute respiratory syndrome-related coronavirus | ||
| GSM4592631 | hESC-derived cardiomyocytes_SARS-CoV-2_3 | cardiomyocyte cell line |
SARS-CoV-2 | Severe acute respiratory syndrome-related coronavirus | ||
| GSM4592632 | Adult human cardiomyocytes_Mock_1 | cardiomyocyte cell line |
Mock | Uninfected | ||
| GSM4592633 | Adult human cardiomyocytes_Mock_2 | cardiomyocyte cell line |
Mock | Uninfected | ||
| GSM4592634 | Adult human cardiomyocytes_Mock_3 | cardiomyocyte cell line |
Mock | Uninfected | ||
| GSM4592635 | Adult human cardiomyocytes_SARS-CoV-2_1 | cardiomyocyte cell line |
SARS-CoV-2 | Severe acute respiratory syndrome-related coronavirus | ||
| GSM4592636 | Adult human cardiomyocytes_SARS-CoV-2_2 | cardiomyocyte cell line |
SARS-CoV-2 | Severe acute respiratory syndrome-related coronavirus | ||
| GSM4592637 | Adult human cardiomyocytes_SARS-CoV-2_3 | cardiomyocyte cell line |
SARS-CoV-2 | Severe acute respiratory syndrome-related coronavirus | ||
| GSM4592638 | Macrophages_Mock_1 | macrophage |
Mock | Uninfected | ||
| GSM4592639 | Macrophages_Mock_2 | macrophage |
Mock | Uninfected | ||
| GSM4592640 | Macrophages_Mock_3 | macrophage |
Mock | Uninfected | ||
| GSM4592641 | Macrophages_SARS-CoV-2_1 | macrophage |
SARS-CoV-2 | Severe acute respiratory syndrome-related coronavirus | ||
| GSM4592642 | Macrophages_SARS-CoV-2_2 | macrophage |
SARS-CoV-2 | Severe acute respiratory syndrome-related coronavirus | ||
| GSM4592643 | Macrophages_SARS-CoV-2_3 | macrophage |
SARS-CoV-2 | Severe acute respiratory syndrome-related coronavirus |