GSE153218

Bulk RNA sequencing of SARS-CoV-2 infected alveolar type I- and type II like cells

Submitted by Jelte van der Vaart (Hubrecht Institute, Netherlands) on Jun 25 2020

Platform: ngs – Illumina NextSeq 500 (Homo sapiens)

Pubmed: 33283287

Summary Bulk RNA sequencing was performed on control and SARS-CoV-2 infected 2D bronchioalveolar-like and small airway cultures

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), may result in acute respiratory distress syndrome (ARDS), multi-organ failure and death. The alveolar epithelium is a major target of the virus, but representative models to study virus host interactions in more detail are currently lacking. Here, we describe a human 2D air-liquid interphase culture system which was characterized by confocal-, electron-microscopy and mRNA expression analysis. In this model, alveolar type I- (ATI-L) and type II-like (ATII-L) cells, but also basal cells, neuroendocrine cells and club cells, are grown from 3D self-renewing lung bud tip organoids. These cultures were readily infected by SARS-CoV-2 with both ATI-L and ATII-L cells being targeted. Consequently, significant viral titers were detected and mRNA expression analysis revealed induction of a type I/III interferon response program. Hence, these cultures represent an experimental model for SARS-CoV-2 infection and can be applied for drug screens.

16 Samples

ID	Title	Cell Type	Timepoint	Reported Virus	Virus Species
GSM4635978	Bronchioalveo_1_mock_1	bronchial epithelial cell line		Mock	Uninfected
GSM4635979	Bronchioalveo_1_mock_2	bronchial epithelial cell line		Mock	Uninfected
GSM4635980	Bronchioalveo_1_SARS-CoV-2_1	bronchial epithelial cell line	72 hrs	SARS-CoV-2	Severe acute respiratory syndrome-related coronavirus
GSM4635981	Bronchioalveo_1_SARS-CoV-2_2	bronchial epithelial cell line	72 hrs	SARS-CoV-2	Severe acute respiratory syndrome-related coronavirus
GSM4635982	Bronchioalveo_2_mock_1	bronchial epithelial cell line		Mock	Uninfected
GSM4635983	Bronchioalveo_2_mock_2	bronchial epithelial cell line		Mock	Uninfected
GSM4635984	Bronchioalveo_2_SARS-CoV-2_1	bronchial epithelial cell line		SARS-CoV-2	Severe acute respiratory syndrome-related coronavirus
GSM4635985	Bronchioalveo_2_SARS-CoV-2_2	bronchial epithelial cell line		SARS-CoV-2	Severe acute respiratory syndrome-related coronavirus
GSM4635986	SmallAirway_1_mock	SAEC cell		Mock	Uninfected
GSM4635987	SmallAirway_2_mock	SAEC cell		Mock	Uninfected
GSM4635988	SmallAirway_1_SARS-CoV-2	SAEC cell		SARS-CoV-2	Severe acute respiratory syndrome-related coronavirus
GSM4635989	SmallAirway_2_SARS-CoV-2	SAEC cell		SARS-CoV-2	Severe acute respiratory syndrome-related coronavirus
GSM4635990	fetal3D1	lung bud		Mock	Uninfected
GSM4635991	fetal3D2	lung bud		Mock	Uninfected
GSM4635992	nonALI1	lung bud		Mock	Uninfected
GSM4635993	nonALI2	lung bud		Mock	Uninfected