Virus Expression Database

GSE154998

Transcriptional Profiling of Leukocytes in Critically Ill COVID19 Patients

Submitted by David E Carter (Robarts Research Institute, Canada) on Jul 23 2020

Platform: ngs – Illumina NextSeq 500 (Homo sapiens)

Pubmed: 33306162

Summary Objective: COVID19 is caused by the SARS-CoV-2 virus and has been associated with severe inflammation leading to organ dysfunction and mortality. Our aim was to profile the transcriptome in leukocytes from critically ill ICU patients positive for COVID19 vs. those negative for COVID19 to better understand the COVID19 associated host response.
Design: Transcriptome profiling of buffy coat cells via ribonucleic acid sequencing (RNAseq) at the time of admission to the ICU.
Setting: Tertiary care ICU and academic laboratory.
Subjects: All patients admitted to the ICU suspected of being infected with SARS-CoV-2, using standardized hospital screening methodologies, had blood samples collected at the time of admission to the ICU.
Interventions: None.
Measurement and Main Results: Age- and sex-matched ICU patients that were either COVID19+ (PCR positive, 2 genes) or COVID19- (PCR negative) were enrolled. Cohorts were well-balanced with the exception that COVID19- patients had significantly higher total white blood cell counts and circulating neutrophils and COVID19+ patients were more likely to suffer bilateral pneumonia compared to COVID19- patients. Further, the mortality rate for this cohort of COVID19+ ICU patients was 29%. Transcriptional analysis revealed that when compared to COVID19- patients, the altered transcriptional responses of leukocytes in critically ill COVID19+ ICU patients appeared to be associated with multiple interrelated outcomes, including but not limited to robust interferon (IFN)-associated transcriptional responses, a marked decrease in the transcriptional activity of genes contributing to protein synthesis and the dysregulated expression of genes that contribute to coagulation, platelet activation, Toll-like receptor activation, neurotrophin signaling, and protein SUMOylation/ubiquitination.
Conclusions: COVID19+ patients on day 1 of admission to the ICU display a unique leukocyte transcriptional profile that distinguishes them from COVID19- patients. Identification of this profile provides guidance for future targeted studies exploring novel prognostic/therapeutic aspects of COVID19.

14 Samples

ID Title Cell Type Timepoint Reported Virus Virus Species Exclusion Reason
GSM4692990 Buffy coat cells COVID- Rep1 buffy coat cell  Healthy Uninfected
GSM4692991 Buffy coat cells COVID+ Rep1 buffy coat cell  SARS-CoV-2 Severe acute respiratory syndrome-related coronavirus
GSM4692992 Buffy coat cells COVID- Rep2 buffy coat cell  Healthy Uninfected
GSM4692993 Buffy coat cells COVID- Rep3 buffy coat cell  Healthy Uninfected
GSM4692994 Buffy coat cells COVID+ Rep2 buffy coat cell  SARS-CoV-2 Severe acute respiratory syndrome-related coronavirus
GSM4692995 Buffy coat cells COVID- Rep4 buffy coat cell  Healthy Uninfected
GSM4692996 Buffy coat cells COVID+ Rep3 buffy coat cell  SARS-CoV-2 Severe acute respiratory syndrome-related coronavirus
GSM4692997 Buffy coat cells COVID- Rep5 buffy coat cell  Healthy Uninfected
GSM4692998 Buffy coat cells COVID+ Rep4 buffy coat cell  SARS-CoV-2 Severe acute respiratory syndrome-related coronavirus
GSM4692999 Buffy coat cells COVID+ Rep5 buffy coat cell  SARS-CoV-2 Severe acute respiratory syndrome-related coronavirus
GSM4693000 Buffy coat cells COVID- Rep6 buffy coat cell  Healthy Uninfected
GSM4693001 Buffy coat cells COVID- Rep7 buffy coat cell  Healthy Uninfected
GSM4693002 Buffy coat cells COVID+ Rep6 buffy coat cell  SARS-CoV-2 Severe acute respiratory syndrome-related coronavirus
GSM4693003 Buffy coat cells COVID+ Rep7 buffy coat cell  SARS-CoV-2 Severe acute respiratory syndrome-related coronavirus