Virus Expression Database

GSE17908

Differential gene expression patterns of EBV infected EBNA-3 positive and negative human B lymphocytes

Submitted by Roland Lang (University Hospital Erlangen, Germany) on Sep 01 2009

Platform: microarray – [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array

Pubmed: 19578441

Summary The genome of Epstein-Barr virus (EBV) encodes 86 proteins but only a limited set is expressed in EBV-growth transformed B cells, termed lymphoblastoid cell lines (LCLs). These cells proliferate via the concerted action of EBV nuclear antigens (EBNAs) and latent membrane proteins (LMPs), some of which are rate limiting to establish a stable homeostasis of growth promoting and anti-apoptotic activities. We show here that EBV mutants, which lack the EBNA-3A gene, are impaired but can still initiate cell-cycle entry and proliferation of primary human B cells in contrast to an EBNA-2-deficient mutant virus. Surprisingly and in contrast to previous reports, these viral mutants are attenuated in growth transformation assays but give rise to permanently growing EBNA-3A negative B cell lines which exhibit reduced proliferation rates and elevated levels of apoptosis. Expression profiles of EBNA-3A deficient LCLs are characterized by 129 upregulated and 167 downregulated genes, which are significantly enriched for genes involved in apoptotic processes or cell cycle progression like the tumor suppressor gene p16/INK4A or might contribute to essential steps in the viral life cycle. In addition EBNA-3A cellular target genes remarkably overlap with previously identified targets of EBNA-2.

5 Samples

ID Title Cell Type Timepoint Reported Virus Virus Species Exclusion Reason
GSM447306 Donor 1, EBVwt infected LCL lymphoblastoid cell line  EBV Human gammaherpesvirus 4
GSM447307 Donor 2, EBVwt infected LCL, line 1 lymphoblastoid cell line  EBV Human gammaherpesvirus 4
GSM447308 Donor 2, EBVwt infected LCL, line 2 lymphoblastoid cell line  EBV Human gammaherpesvirus 4
GSM447309 Donor 3, EBVwt infected LCL, line 1 lymphoblastoid cell line  EBV Human gammaherpesvirus 4
GSM447310 Donor 3, EBVwt infected LCL, line 2 lymphoblastoid cell line  EBV Human gammaherpesvirus 4