GSE19761

Expression data from Epstein-Barr virus -positive and negative Akata Burkitt's Lymphoma Clones

Submitted by Rona S Scott (Louisiana State University Health Sciences Center-Shreveport, USA) on Jan 06 2010

Platform: microarray – [HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array

Pubmed: 23891576

Summary Transition of Akata Burkitt lymphoma (BL) cells from a malignant to nonmalignant phenotype upon loss of Epstein-Barr virus (EBV) genomes in vitro is evidence for a viral contribution to tumor maintenance despite the tightly restricted pattern of EBV gene expression in BL. Akata cells retaining virus manifest increased resistance to apoptosis under growth limiting conditions, although ambiguity exists regarding the exact mechanisms involved. By examining global cellular gene expression differences in Akata subclones that had either retained or lost EBV, we identified spermidine/spermine N1-acetyltransferase (SSAT), an inducible acetylating enzyme whose catabolism of polyamines affects both apoptosis and cell growth, as one of a limited number of cellular genes up-regulated upon loss of EBV.

Keywords: Disease state analysis

6 Samples

ID	Title	Cell Type	Timepoint	Reported Virus	Virus Species	Exclusion Reason
GSM493574	2A8 EBV-negative Akata BL clone,4hr starve, biological rep 1	Burkitt lymphoma cell line  [Akata ]		none	Uninfected
GSM493575	2A8 EBV-negative Akata BL clone, 4hr starve, biological rep 2	Burkitt lymphoma cell line  [Akata ]		none	Uninfected
GSM493576	2A8 EBV-negative Akata BL clone, 4hr starve, biological rep 3	Burkitt lymphoma cell line  [Akata ]		none	Uninfected
GSM493577	1B6 EBV-positive Akata BL clone, 4hr starve, biological rep 1	Burkitt lymphoma cell line  [Akata ]		EBV	Human gammaherpesvirus 4
GSM493578	1B6 EBV-positive Akata BL clone, 4hr starve, biological rep 2	Burkitt lymphoma cell line  [Akata ]		EBV	Human gammaherpesvirus 4
GSM493579	1B6 EBV-positive Akata BL clone, 4hr starve, biological rep 3	Burkitt lymphoma cell line  [Akata ]		EBV	Human gammaherpesvirus 4