Virus Expression Database
Submitted by Kory R Johnson (NINDS NIH, USA) on Jan 28 2013
Platform: microarray – [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Pubmed: 23536657
Summary Viral infections of the CNS are of increasing concern, especially among immunocompromised populations. Rodent models are often inappropriate for studies of CNS infection, as many viruses, including JC Virus (JCV) and HIV, cannot replicate in rodent cells. Consequently, human fetal brain-derived multipotential CNS progenitor cells (NPCs) that can be differentiated into neurons, oligodendrocytes, or astrocytes, have served as a model for CNS studies. NPCs can be non-productively infected by JCV, while infection of progenitor-derived astrocytes (PDAs) is robust. We profiled cellular gene expression at multiple times during differentiation of NPCs to PDAs. Several activated transcription factors show commonality between cells of the brain in which JCV replicates and lymphocytes in which JCV is likely latent. Bioinformatic analysis determined transcription factors that may influence the favorable transcriptional environment for JCV in PDAs. This study attempts to provide a framework for understanding the functional transcriptional profile necessary for productive JCV infection.
| ID | Title | Cell Type | Timepoint | Reported Virus | Virus Species | Exclusion Reason |
|---|---|---|---|---|---|---|
| GSM1071337 | Human Fetal Brain NPC 0h Replicate 1 | neural stem cell  |
JCV | Uninfected | ||
| GSM1071338 | Human Fetal Brain NPC 0h Replicate 2 | neural stem cell |
JCV | Uninfected | ||
| GSM1071339 | Human Fetal Brain NPC 0h Replicate 3 | neural stem cell |
JCV | Uninfected | ||
| GSM1071340 | Human Fetal Brain NPC 0h Replicate 4 | neural stem cell |
JCV | Uninfected | ||
| GSM1071341 | Human Fetal Brain NPC in Serum 1h Replicate 1 | neural stem cell |
1 hr | JCV | Human polyomavirus 2 | |
| GSM1071342 | Human Fetal Brain NPC in Serum 1h Replicate 2 | neural stem cell |
1 hr | JCV | Human polyomavirus 2 | |
| GSM1071343 | Human Fetal Brain NPC in Serum 1h Replicate 3 | neural stem cell |
1 hr | JCV | Human polyomavirus 2 | |
| GSM1071344 | Human Fetal Brain NPC in Serum 1h Replicate 4 | neural stem cell |
1 hr | JCV | Human polyomavirus 2 | |
| GSM1071345 | Human Fetal Brain NPC in Serum 1d Replicate 1 | neural stem cell |
1 day | JCV | Human polyomavirus 2 | |
| GSM1071346 | Human Fetal Brain NPC in Serum 1d Replicate 2 | neural stem cell |
1 day | JCV | Human polyomavirus 2 | |
| GSM1071347 | Human Fetal Brain NPC in Serum 1d Replicate 3 | neural stem cell |
1 day | JCV | Human polyomavirus 2 | |
| GSM1071348 | Human Fetal Brain NPC in Serum 1d Replicate 4 | neural stem cell |
1 day | JCV | Human polyomavirus 2 | |
| GSM1071349 | Human Fetal Brain NPC in Serum 7d Replicate 1 | neural stem cell |
7 days | JCV | Human polyomavirus 2 | |
| GSM1071350 | Human Fetal Brain NPC in Serum 7d Replicate 2 | neural stem cell |
7 days | JCV | Human polyomavirus 2 | |
| GSM1071351 | Human Fetal Brain NPC in Serum 7d Replicate 3 | neural stem cell |
7 days | JCV | Human polyomavirus 2 | |
| GSM1071352 | Human Fetal Brain NPC in Serum 7d Replicate 4 | neural stem cell |
7 days | JCV | Human polyomavirus 2 | |
| GSM1071353 | Human Fetal Brain NPC in Serum 30d Replicate 1 | neural stem cell |
30 days | JCV | Human polyomavirus 2 | |
| GSM1071354 | Human Fetal Brain NPC in Serum 30d Replicate 2 | neural stem cell |
30 days | JCV | Human polyomavirus 2 | |
| GSM1071355 | Human Fetal Brain NPC in Serum 30d Replicate 3 | neural stem cell |
30 days | JCV | Human polyomavirus 2 |