GSE45639

Clonal Immortalized Human Glial Cell Lines Support Varying Levels of JC Virus Infection due to Differences in Cellular Gene Expression

Submitted by Kory R Johnson (NINDS NIH, USA) on Mar 29 2013

Platform: microarray – [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]

Pubmed: 24052414

Summary JC virus (JCV) is a ubiquitous human polyomavirus that causes the demyelinating disease Progressive Multifocal Leukoencephalopathy (PML). JCV replicates in limited cell types in culture, predominantly in human glial cells. Thus, productive JCV infection is an indicator of the host cell transcription environment. Following introduction of a replication defective SV40 mutant that expressed large T protein into a heterogeneous culture of human fetal brain cells, multiple phenotypes became immortalized (SVG cells). A subset of SVG cells could support JCV replication. This mixed culture was called SVG cells. In the current study, clonal cell lines were selected from the original SVG cell culture. The SVG-5F4 clone showed low levels of viral growth. The SVG-10B1 clone was highly permissive for JCV DNA replication and gene expression. Microarray analysis revealed that viral infection did not significantly change gene expression in these cells. More resistant 5F4 cells expressed high levels of transcription factors known to inhibit JCV transcription. Interestingly, 5F4 cells highly expressed RNA of markers of Bergman or radial glia and 10B1 cells had high expression of markers of immature glial cells and activation of transcription regulators important for stem/progenitor cell self-renewal. These SVG-derived clonal cell lines provide a biologically relevant model to investigate cell type differences in JCV host range and pathogenesis, as well as neural development.

13 Samples

ID	Title	Cell Type	Timepoint	Reported Virus	Virus Species
GSM1111182	SVG 10B1 Clone Mock Infected Replicate 1	glial cell line [SVG ]	14 days	mock	Uninfected
GSM1111183	SVG 10B1 Clone Mock Infected Replicate 2	glial cell line [SVG ]	14 days	mock	Uninfected
GSM1111184	SVG 10B1 Clone Mock Infected Replicate 3	glial cell line [SVG ]	14 days	mock	Uninfected
GSM1111185	SVG 10B1 Clone JCV Mad-4 Strain Infected Replicate 1	glial cell line [SVG ]	14 days	JCV Mad-4	Human polyomavirus 2
GSM1111186	SVG 10B1 Clone JCV Mad-4 Strain Infected Replicate 2	glial cell line [SVG ]	14 days	JCV Mad-4	Human polyomavirus 2
GSM1111187	SVG 10B1 Clone JCV Mad-4 Strain Infected Replicate 3	glial cell line [SVG ]	14 days	JCV Mad-4	Human polyomavirus 2
GSM1111188	SVG 5F4 Clone Mock Infected Replicate 1	glial cell line [SVG ]	14 days	mock	Uninfected
GSM1111189	SVG 5F4 Clone Mock Infected Replicate 2	glial cell line [SVG ]	14 days	mock	Uninfected
GSM1111190	SVG 5F4 Clone Mock Infected Replicate 3	glial cell line [SVG ]	14 days	mock	Uninfected
GSM1111191	SVG 5F4 Clone JCV Mad-4 Strain Infected Replicate 1	glial cell line [SVG ]	14 days	JCV Mad-4	Human polyomavirus 2
GSM1111192	SVG 5F4 Clone JCV Mad-4 Strain Infected Replicate 2	glial cell line [SVG ]	14 days	JCV Mad-4	Human polyomavirus 2
GSM1111193	SVG 5F4 Clone JCV Mad-4 Strain Infected Replicate 3	glial cell line [SVG ]	14 days	JCV Mad-4	Human polyomavirus 2
GSM1111194	SVG 5F4 Clone JCV Mad-4 Strain Infected Replicate 4	glial cell line [SVG ]	14 days	JCV Mad-4	Human polyomavirus 2