Submitted by Neelanjan Mukherjee (MDC Berlin, Germany) on May 03 2013
Platform: ngs – Illumina HiSeq 2000 (Homo sapiens)
Pubmed: 23708386
Summary High-throughput sequencing has opened numerous possibilities for the identification of regulatory RNA-binding events. Cross-linking and immunoprecipitation of Argonaute protein members can pinpoint microRNA target sites within tens of bases, but leaves the identity of the microRNA unresolved. A flexible computational framework that integrates sequence with cross-linking features reliably identifies the microRNA family involved in each binding event, considerably outperforms sequence-only approaches, and quantifies the prevalence of noncanonical binding modes.
ID | Title | Cell Type | Timepoint | Reported Virus | Virus Species | Exclusion Reason |
---|---|---|---|---|---|---|
GSM1133247 | LCLBACWT | lymphoblastoid cell line ![]() |
4 mos | EBV | Human gammaherpesvirus 4 | |
GSM1133248 | LCLBACD1_1 | lymphoblastoid cell line ![]() |
4 mos | EBV | Human gammaherpesvirus 4 | Extra interventions EBV missing a mir |
GSM1133249 | LCLBACD1_2 | lymphoblastoid cell line ![]() |
4 mos | EBV | Human gammaherpesvirus 4 | Extra interventions EBV missing a mir |
GSM1133250 | LCLBACD2 | lymphoblastoid cell line ![]() |
4 mos | EBV | Human gammaherpesvirus 4 | Extra interventions EBV missing a mir |
GSM1133251 | LCLBACD3 | lymphoblastoid cell line ![]() |
4 mos | EBV | Human gammaherpesvirus 4 | Extra interventions EBV missing a mir |