Virus Expression Database
Submitted by Valerio Licursi (Università di Roma "Sapienza", Italy) on May 21 2013
Platform: microarray – [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Pubmed: 23922808
Summary With the goal of specifically dissecting the toxicogenomic signatures of the helper-dependent (HD) human (HAd5) and canine (CAV-2) adenovirus, the VSV-G-pseudotyped SIN HIV-1 (LV) and the Adenoviral-associated vector 2/9 for human neurons (AAV2/9), we transduced a bona fide human neuronal system with HD-HAd5, HD-CAV-2, LV and AAV2/9, we analysed the transcriptional response of more than 47,000 transcripts using gene chips.
Chip data showed that HD-CAV-2 and LV vectors both activated the innate arm of the immune response, including Toll-like receptors and hyaluronan circuits. LV vector induced as well an IFN response. Moreover, HD-CAV-2 and LV vectors affected DNA damage pathways - at 5 days in opposite directions - suggesting a differential response of the p53 and ATM pathways to the vector genomes. As a general response to the vectors, human neurons activated pro-survival genes and neuron morphogenesis.
| ID | Title | Cell Type | Timepoint | Reported Virus | Virus Species | Exclusion Reason |
|---|---|---|---|---|---|---|
| GSM1145487 | hmNPCs_untreated_2h_rep1 | neural stem cell  |
2 hrs | untreated | Uninfected | |
| GSM1145488 | hmNPCs_untreated_2h_rep2 | neural stem cell |
2 hrs | untreated | Uninfected | |
| GSM1145489 | hmNPCs_untreated_2h_rep3 | neural stem cell |
2 hrs | untreated | Uninfected | |
| GSM1145490 | hmNPCs_untreated_5d_rep1 | neural stem cell |
5 days | untreated | Uninfected | |
| GSM1145491 | hmNPCs_untreated_5d_rep2 | neural stem cell |
5 days | untreated | Uninfected | |
| GSM1145492 | hmNPCs_untreated_5d_rep3 | neural stem cell |
5 days | untreated | Uninfected | |
| GSM1145499 | hmNPCs_HD-HAd_2h_rep1 | neural stem cell |
2 hrs | HD-HAd5 | Human mastadenovirus C | Extra interventions helper-dependent virus |
| GSM1145500 | hmNPCs_HD-HAd_2h_rep2 | neural stem cell |
2 hrs | HD-HAd5 | Human mastadenovirus C | Extra interventions helper-dependent virus |
| GSM1145501 | hmNPCs_HD-HAd_2h_rep3 | neural stem cell |
2 hrs | HD-HAd5 | Human mastadenovirus C | Extra interventions helper-dependent virus |
| GSM1145502 | hmNPCs_HD-HAd_5d_rep1 | neural stem cell |
5 days | HD-HAd5 | Human mastadenovirus C | Extra interventions helper-dependent virus |
| GSM1145503 | hmNPCs_HD-HAd_5d_rep2 | neural stem cell |
5 days | HD-HAd5 | Human mastadenovirus C | Extra interventions helper-dependent virus |
| GSM1145504 | hmNPCs_HD-HAd_5d_rep3 | neural stem cell |
5 days | HD-HAd5 | Human mastadenovirus C | Extra interventions helper-dependent virus |
| GSM1145511 | hmNPCs_AAV2/9_2h_rep1 | neural stem cell |
2 hrs | AAV2/9 | Adeno-associated dependoparvovirus A | |
| GSM1145512 | hmNPCs_AAV2/9_2h_rep2 | neural stem cell |
2 hrs | AAV2/9 | Adeno-associated dependoparvovirus A | |
| GSM1145513 | hmNPCs_AAV2/9_2h_rep3 | neural stem cell |
2 hrs | AAV2/9 | Adeno-associated dependoparvovirus A | |
| GSM1145514 | hmNPCs_AAV2/9_5d_rep1 | neural stem cell |
5 days | AAV2/9 | Adeno-associated dependoparvovirus A | |
| GSM1145515 | hmNPCs_AAV2/9_5d_rep2 | neural stem cell |
5 days | AAV2/9 | Adeno-associated dependoparvovirus A | |
| GSM1145516 | hmNPCs_AAV2/9_5d_rep3 | neural stem cell |
5 days | AAV2/9 | Adeno-associated dependoparvovirus A |