Submitted by Dominik Lutter (Helmholtz Zentrum München, Germany) on Jan 08 2014
Platform: microarray – [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Pubmed: 25948739
Summary LMP2A of Epstein-Barr virus is a receptor that mimics an activated B cell receptor, BCR. K1 and K15, related receptors of Kaposi sarcoma-associated herpes virus, KSHV, are expressed in virus-associated tumors but their functions are less obvious. We addressed this uncertainty with mutant EBVs encoding the KSHV genes K1 or K15 in lieu of LMP2A and infected primary human B cells with them. K1 and K15 encoded proteins appear to have noncomplementing redundant functions in this model but our findings suggest that both KSHV proteins can replace LMP2A’s key activities contributing to the survival, activation and proliferation of B cells.
ID | Title | Cell Type | Timepoint | Reported Virus | Virus Species | Exclusion Reason |
---|---|---|---|---|---|---|
GSM1303476 | B-cell_wt_EBV_rep1 | B-lymphocyte ![]() |
8 days | EBV | Human gammaherpesvirus 4 | |
GSM1303477 | B-cell_wt_EBV_rep2 | B-lymphocyte ![]() |
8 days | EBV | Human gammaherpesvirus 4 | |
GSM1303478 | B-cell_wt_EBV_rep3 | B-lymphocyte ![]() |
8 days | EBV | Human gammaherpesvirus 4 | |
GSM1303479 | B-cell_wt_EBV_rep4 | B-lymphocyte ![]() |
8 days | EBV | Human gammaherpesvirus 4 | |
GSM1303480 | B-cell_LMP2A_EBV_rep1 | B-lymphocyte ![]() |
8 days | EBV-dLMP2A | Human gammaherpesvirus 4 | Extra interventions EBV with deleted LMP2A |
GSM1303481 | B-cell_LMP2A_EBV_rep2 | B-lymphocyte ![]() |
8 days | EBV-dLMP2A | Human gammaherpesvirus 4 | Extra interventions EBV with deleted LMP2A |
GSM1303482 | B-cell_LMP2A_EBV_rep3 | B-lymphocyte ![]() |
8 days | EBV-dLMP2A | Human gammaherpesvirus 4 | Extra interventions EBV with deleted LMP2A |
GSM1303483 | B-cell_LMP2A_EBV_rep4 | B-lymphocyte ![]() |
8 days | EBV-dLMP2A | Human gammaherpesvirus 4 | Extra interventions EBV with deleted LMP2A |
GSM1303484 | B-cell_K1_EBV_rep1 | B-lymphocyte ![]() |
8 days | EBV-K1 | Human gammaherpesvirus 4 | Extra interventions EBV with KSHV K1 |
GSM1303485 | B-cell_K1_EBV_rep2 | B-lymphocyte ![]() |
8 days | EBV-K1 | Human gammaherpesvirus 4 | Extra interventions EBV with KSHV K1 |
GSM1303486 | B-cell_K1_EBV_rep3 | B-lymphocyte ![]() |
8 days | EBV-K1 | Human gammaherpesvirus 4 | Extra interventions EBV with KSHV K1 |
GSM1303487 | B-cell_K1_EBV_rep4 | B-lymphocyte ![]() |
8 days | EBV-K1 | Human gammaherpesvirus 4 | Extra interventions EBV with KSHV K1 |
GSM1303488 | B-cell_K15_EBV_rep1 | B-lymphocyte ![]() |
8 days | EBV-K15 | Human gammaherpesvirus 4 | Extra interventions EBV with KSHV K15 |
GSM1303489 | B-cell_K15_EBV_rep2 | B-lymphocyte ![]() |
8 days | EBV-K15 | Human gammaherpesvirus 4 | Extra interventions EBV with KSHV K15 |
GSM1303490 | B-cell_K15_EBV_rep3 | B-lymphocyte ![]() |
8 days | EBV-K15 | Human gammaherpesvirus 4 | Extra interventions EBV with KSHV K15 |
GSM1303491 | B-cell_K15_EBV_rep4 | B-lymphocyte ![]() |
8 days | EBV-K15 | Human gammaherpesvirus 4 | Extra interventions EBV with KSHV K15 |