Virus Expression Database

GSE56192

Transcriptomic analysis of the Novel Middle East Respiratory Syndrome Coronavirus (Human, MRC5 cells)

Submitted by David A Rasko (University of Maryland School of Medicine, USA) on May 16 2014

Platform: ngs – Illumina HiSeq 2000 (Homo sapiens)

Pubmed: None listed

Summary We will use the EMC/2012 strain of the novel beta Coronavirus called Middle East Respiratory Syndrome Coronavirus (MERS-CoV). It was initially passaged on Vero E6 cells in Saudi Arabia before being sequenced at the Erasmus Medical College in Rotterdam, Netherlands by Dr Ron Fouchier. We propose to perform a time course of infection of hCoV-EMC on MRC5 cells (Human Lung origin) and Vero cells (African Green Monkey Kidney cells). Both cell lines readily grow and replicate the virus. Importantly these cell lines show signs of Cytopathic effect (CPE), such as cell rounding and release from the petri dish that coincide with time points high virus replication demonstrating the effects of virus replication on the cells. Transcriptomic analysis will be performed after infection with MERS-CoV and SARS-CoV (Urbani strain) to compare the host gene induction that occurs during infection.

37 Samples

ID Title Cell Type Timepoint Reported Virus Virus Species Exclusion Reason
GSM1387907 MOCK_MRC5_1 lung fibroblast cell line  [MRC-5 ] mock Uninfected
GSM1387908 MOCK_MRC5_2 lung fibroblast cell line  [MRC-5 ] mock Uninfected
GSM1387909 MOCK_MRC5_3 lung fibroblast cell line  [MRC-5 ] mock Uninfected
GSM1387910 MERS_MRC5lowMOI_24hr_1 lung fibroblast cell line  [MRC-5 ] 24 hrs MERS Middle East respiratory syndrome-related coronavirus
GSM1387911 MERS_MRC5lowMOI_24hr_2 lung fibroblast cell line  [MRC-5 ] 24 hrs MERS Middle East respiratory syndrome-related coronavirus
GSM1387912 MERS_MRC5lowMOI_24hr_3 lung fibroblast cell line  [MRC-5 ] 24 hrs MERS Middle East respiratory syndrome-related coronavirus
GSM1387913 MERS_MRC5HighMOI_24hr_1 lung fibroblast cell line  [MRC-5 ] 24 hrs MERS Middle East respiratory syndrome-related coronavirus
GSM1387914 MERS_MRC5HighMOI_24hr_2 lung fibroblast cell line  [MRC-5 ] 24 hrs MERS Middle East respiratory syndrome-related coronavirus
GSM1387915 MERS_MRC5HighMOI_24hr_3 lung fibroblast cell line  [MRC-5 ] 24 hrs MERS Middle East respiratory syndrome-related coronavirus
GSM1387916 MERS_MRC5lowMOI_48hr_1 lung fibroblast cell line  [MRC-5 ] 48 hrs MERS Middle East respiratory syndrome-related coronavirus
GSM1387917 MERS_MRC5lowMOI_48hr_2 lung fibroblast cell line  [MRC-5 ] 48 hrs MERS Middle East respiratory syndrome-related coronavirus
GSM1387918 MERS_MRC5lowMOI_48hr_3 lung fibroblast cell line  [MRC-5 ] 48 hrs MERS Middle East respiratory syndrome-related coronavirus
GSM1387919 MERS_MRC5HighMOI_48hr_1 lung fibroblast cell line  [MRC-5 ] 48 hrs MERS Middle East respiratory syndrome-related coronavirus
GSM1387920 MERS_MRC5HighMOI_48hr_2 lung fibroblast cell line  [MRC-5 ] 48 hrs MERS Middle East respiratory syndrome-related coronavirus
GSM1387921 MERS_MRC5HighMOI_48hr_3 lung fibroblast cell line  [MRC-5 ] 48 hrs MERS Middle East respiratory syndrome-related coronavirus
GSM1387922 SARS_MRC5lowMOI_24hr_1 lung fibroblast cell line  [MRC-5 ] 24 hrs SARS Severe acute respiratory syndrome-related coronavirus
GSM1387923 SARS_MRC5lowMOI_24hr_2 lung fibroblast cell line  [MRC-5 ] 24 hrs SARS Severe acute respiratory syndrome-related coronavirus
GSM1387924 SARS_MRC5lowMOI_24hr_3 lung fibroblast cell line  [MRC-5 ] 24 hrs SARS Severe acute respiratory syndrome-related coronavirus
GSM1387925 SARS_MRC5HighMOI_24hr_1 lung fibroblast cell line  [MRC-5 ] 24 hrs SARS Severe acute respiratory syndrome-related coronavirus
GSM1387926 SARS_MRC5HighMOI_24hr_2 lung fibroblast cell line  [MRC-5 ] 24 hrs SARS Severe acute respiratory syndrome-related coronavirus
GSM1387927 SARS_MRC5HighMOI_24hr_3 lung fibroblast cell line  [MRC-5 ] 24 hrs SARS Severe acute respiratory syndrome-related coronavirus
GSM1387928 SARS_MRC5lowMOI_48hr_2 lung fibroblast cell line  [MRC-5 ] 48 hrs SARS Severe acute respiratory syndrome-related coronavirus
GSM1387929 SARS_MRC5lowMOI_48hr_3 lung fibroblast cell line  [MRC-5 ] 48 hrs SARS Severe acute respiratory syndrome-related coronavirus
GSM1387930 SARS_MRC5HighMOI_48hr_2 lung fibroblast cell line  [MRC-5 ] 48 hrs SARS Severe acute respiratory syndrome-related coronavirus
GSM1387931 SARS_MRC5HighMOI_48hr_3 lung fibroblast cell line  [MRC-5 ] 48 hrs SARS Severe acute respiratory syndrome-related coronavirus
GSM1387932 MOCK_MRC5_IFNbeta_24hrs lung fibroblast cell line  [MRC-5 ] 24 hrs mock Uninfected Extra interventions
IFNbeta
GSM1387933 MERS_MRC5_IFNbeta_24hrs lung fibroblast cell line  [MRC-5 ] 24 hrs MERS Middle East respiratory syndrome-related coronavirus Extra interventions
IFNbeta
GSM1387934 MOCK_MRC5_Gleevec_24hrs lung fibroblast cell line  [MRC-5 ] 24 hrs mock Uninfected Extra interventions
Gleevec
GSM1387935 MERS_MRC5_Gleevec_24hrs lung fibroblast cell line  [MRC-5 ] 24 hrs MERS Middle East respiratory syndrome-related coronavirus Extra interventions
Gleevec
GSM1387936 MOCK_MRC5_IFNbeta_48hrs lung fibroblast cell line  [MRC-5 ] 48 hrs mock Uninfected Extra interventions
IFNbeta
GSM1387937 MERS_MRC5_IFNbeta_48hrs lung fibroblast cell line  [MRC-5 ] 48 hrs MERS Middle East respiratory syndrome-related coronavirus Extra interventions
IFNbeta
GSM1387938 MOCK_MRC5_Gleevec_48hrs lung fibroblast cell line  [MRC-5 ] 48 hrs mock Uninfected Extra interventions
Gleevec
GSM1387939 MERS_MRC5_Gleevec_48hrs lung fibroblast cell line  [MRC-5 ] 48 hrs MERS Middle East respiratory syndrome-related coronavirus Extra interventions
Gleevec
GSM1387940 SARS_MRC5_IFNbeta_24hrs lung fibroblast cell line  [MRC-5 ] 24 hrs SARS Severe acute respiratory syndrome-related coronavirus Extra interventions
IFNbeta
GSM1387941 SARS_MRC5_IFNbeta_48hrs lung fibroblast cell line  [MRC-5 ] 48 hrs SARS Severe acute respiratory syndrome-related coronavirus Extra interventions
IFNbeta
GSM1387942 SARS_MRC5_Gleevec_24hrs lung fibroblast cell line  [MRC-5 ] 24 hrs SARS Severe acute respiratory syndrome-related coronavirus Extra interventions
Gleevec
GSM1387943 SARS_MRC5_Gleevec_48hrs lung fibroblast cell line  [MRC-5 ] 48 hrs SARS Severe acute respiratory syndrome-related coronavirus Extra interventions
Gleevec