Virus Expression Database
Submitted by David A Rasko (University of Maryland School of Medicine, USA) on May 16 2014
Platform: ngs – Illumina HiSeq 2000 (Homo sapiens)
Pubmed: None listed
Summary We will use the EMC/2012 strain of the novel beta Coronavirus called Middle East Respiratory Syndrome Coronavirus (MERS-CoV). It was initially passaged on Vero E6 cells in Saudi Arabia before being sequenced at the Erasmus Medical College in Rotterdam, Netherlands by Dr Ron Fouchier. We propose to perform a time course of infection of hCoV-EMC on MRC5 cells (Human Lung origin) and Vero cells (African Green Monkey Kidney cells). Both cell lines readily grow and replicate the virus. Importantly these cell lines show signs of Cytopathic effect (CPE), such as cell rounding and release from the petri dish that coincide with time points high virus replication demonstrating the effects of virus replication on the cells. Transcriptomic analysis will be performed after infection with MERS-CoV and SARS-CoV (Urbani strain) to compare the host gene induction that occurs during infection.
| ID | Title | Cell Type | Timepoint | Reported Virus | Virus Species | Exclusion Reason |
|---|---|---|---|---|---|---|
| GSM1387907 | MOCK_MRC5_1 | lung fibroblast cell line  [MRC-5  ] |
mock | Uninfected | ||
| GSM1387908 | MOCK_MRC5_2 | lung fibroblast cell line [MRC-5 ] |
mock | Uninfected | ||
| GSM1387909 | MOCK_MRC5_3 | lung fibroblast cell line [MRC-5 ] |
mock | Uninfected | ||
| GSM1387910 | MERS_MRC5lowMOI_24hr_1 | lung fibroblast cell line [MRC-5 ] |
24 hrs | MERS | Middle East respiratory syndrome-related coronavirus | |
| GSM1387911 | MERS_MRC5lowMOI_24hr_2 | lung fibroblast cell line [MRC-5 ] |
24 hrs | MERS | Middle East respiratory syndrome-related coronavirus | |
| GSM1387912 | MERS_MRC5lowMOI_24hr_3 | lung fibroblast cell line [MRC-5 ] |
24 hrs | MERS | Middle East respiratory syndrome-related coronavirus | |
| GSM1387913 | MERS_MRC5HighMOI_24hr_1 | lung fibroblast cell line [MRC-5 ] |
24 hrs | MERS | Middle East respiratory syndrome-related coronavirus | |
| GSM1387914 | MERS_MRC5HighMOI_24hr_2 | lung fibroblast cell line [MRC-5 ] |
24 hrs | MERS | Middle East respiratory syndrome-related coronavirus | |
| GSM1387915 | MERS_MRC5HighMOI_24hr_3 | lung fibroblast cell line [MRC-5 ] |
24 hrs | MERS | Middle East respiratory syndrome-related coronavirus | |
| GSM1387916 | MERS_MRC5lowMOI_48hr_1 | lung fibroblast cell line [MRC-5 ] |
48 hrs | MERS | Middle East respiratory syndrome-related coronavirus | |
| GSM1387917 | MERS_MRC5lowMOI_48hr_2 | lung fibroblast cell line [MRC-5 ] |
48 hrs | MERS | Middle East respiratory syndrome-related coronavirus | |
| GSM1387918 | MERS_MRC5lowMOI_48hr_3 | lung fibroblast cell line [MRC-5 ] |
48 hrs | MERS | Middle East respiratory syndrome-related coronavirus | |
| GSM1387919 | MERS_MRC5HighMOI_48hr_1 | lung fibroblast cell line [MRC-5 ] |
48 hrs | MERS | Middle East respiratory syndrome-related coronavirus | |
| GSM1387920 | MERS_MRC5HighMOI_48hr_2 | lung fibroblast cell line [MRC-5 ] |
48 hrs | MERS | Middle East respiratory syndrome-related coronavirus | |
| GSM1387921 | MERS_MRC5HighMOI_48hr_3 | lung fibroblast cell line [MRC-5 ] |
48 hrs | MERS | Middle East respiratory syndrome-related coronavirus | |
| GSM1387922 | SARS_MRC5lowMOI_24hr_1 | lung fibroblast cell line [MRC-5 ] |
24 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | |
| GSM1387923 | SARS_MRC5lowMOI_24hr_2 | lung fibroblast cell line [MRC-5 ] |
24 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | |
| GSM1387924 | SARS_MRC5lowMOI_24hr_3 | lung fibroblast cell line [MRC-5 ] |
24 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | |
| GSM1387925 | SARS_MRC5HighMOI_24hr_1 | lung fibroblast cell line [MRC-5 ] |
24 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | |
| GSM1387926 | SARS_MRC5HighMOI_24hr_2 | lung fibroblast cell line [MRC-5 ] |
24 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | |
| GSM1387927 | SARS_MRC5HighMOI_24hr_3 | lung fibroblast cell line [MRC-5 ] |
24 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | |
| GSM1387928 | SARS_MRC5lowMOI_48hr_2 | lung fibroblast cell line [MRC-5 ] |
48 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | |
| GSM1387929 | SARS_MRC5lowMOI_48hr_3 | lung fibroblast cell line [MRC-5 ] |
48 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | |
| GSM1387930 | SARS_MRC5HighMOI_48hr_2 | lung fibroblast cell line [MRC-5 ] |
48 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | |
| GSM1387931 | SARS_MRC5HighMOI_48hr_3 | lung fibroblast cell line [MRC-5 ] |
48 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | |
| GSM1387932 | MOCK_MRC5_IFNbeta_24hrs | lung fibroblast cell line [MRC-5 ] |
24 hrs | mock | Uninfected | Extra interventions IFNbeta |
| GSM1387933 | MERS_MRC5_IFNbeta_24hrs | lung fibroblast cell line [MRC-5 ] |
24 hrs | MERS | Middle East respiratory syndrome-related coronavirus | Extra interventions IFNbeta |
| GSM1387934 | MOCK_MRC5_Gleevec_24hrs | lung fibroblast cell line [MRC-5 ] |
24 hrs | mock | Uninfected | Extra interventions Gleevec |
| GSM1387935 | MERS_MRC5_Gleevec_24hrs | lung fibroblast cell line [MRC-5 ] |
24 hrs | MERS | Middle East respiratory syndrome-related coronavirus | Extra interventions Gleevec |
| GSM1387936 | MOCK_MRC5_IFNbeta_48hrs | lung fibroblast cell line [MRC-5 ] |
48 hrs | mock | Uninfected | Extra interventions IFNbeta |
| GSM1387937 | MERS_MRC5_IFNbeta_48hrs | lung fibroblast cell line [MRC-5 ] |
48 hrs | MERS | Middle East respiratory syndrome-related coronavirus | Extra interventions IFNbeta |
| GSM1387938 | MOCK_MRC5_Gleevec_48hrs | lung fibroblast cell line [MRC-5 ] |
48 hrs | mock | Uninfected | Extra interventions Gleevec |
| GSM1387939 | MERS_MRC5_Gleevec_48hrs | lung fibroblast cell line [MRC-5 ] |
48 hrs | MERS | Middle East respiratory syndrome-related coronavirus | Extra interventions Gleevec |
| GSM1387940 | SARS_MRC5_IFNbeta_24hrs | lung fibroblast cell line [MRC-5 ] |
24 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | Extra interventions IFNbeta |
| GSM1387941 | SARS_MRC5_IFNbeta_48hrs | lung fibroblast cell line [MRC-5 ] |
48 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | Extra interventions IFNbeta |
| GSM1387942 | SARS_MRC5_Gleevec_24hrs | lung fibroblast cell line [MRC-5 ] |
24 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | Extra interventions Gleevec |
| GSM1387943 | SARS_MRC5_Gleevec_48hrs | lung fibroblast cell line [MRC-5 ] |
48 hrs | SARS | Severe acute respiratory syndrome-related coronavirus | Extra interventions Gleevec |