Virus Expression Database

GSE58914

Expression data from Epstein-Barr virus infection of immortalized normal oral keratinocytes

Submitted by Rona S Scott (Louisiana State University Health Sciences Center-Shreveport, USA) on Jun 30 2014

Platform: microarray – [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

Pubmed: 25056883

Summary The oral cavity is the persistent reservoir for EBV with lifelong infection of resident epithelial and B cells. Infection of these cell types results in distinct EBV gene expression patterns that are regulated by epigenetic modifications involving DNA methylation and chromatin structure. Such regulation of EBV gene expression relies on viral manipulation of the host epigenetic machinery that may inadvertently result in long-lasting, oncogenic host epigenetic reprogramming. To test this hypothesis in the context of EBV infection of epithelial cells, we established a transient infection model to identify the epigenetic consequences after EBV infection of immortalized normal oral keratinocytes and subsequent viral loss.
With mounting evidence that EBV can induce epigenetic alterations, we developed a transient infection model where a clonal derivative (designated cl1) from a human telomerase immortalized normal oral keratinocyte (NOK) cell line was infected with a recombinant Akata EBV carrying neomycin resistance and GfP cassette in place of the BXLF1 open reading frame. Infected cells were passaged ten times, and then selection pressure was removed for an additional ten passages to allow for viral loss. Three transiently-infected EBV-negative clones were identified by single cell cloning (designated cl1, cl3, cl4). Uninfected parental clone and cells transfected with PTRUF5 plasmid were passaged alongside the transiently-infected clones. The transcription profiles were analyzed using Affymetrix microarray U133Plus2.0 arrays in duplicate.

12 Samples

ID Title Cell Type Timepoint Reported Virus Virus Species Exclusion Reason
GSM1422231 NOK cl1 uninfected p31 keratinocyte cell line  uninfected Uninfected
GSM1422232 NOK cl1 uninfected p32 keratinocyte cell line  uninfected Uninfected
GSM1422233 NOK cl1 pTURF transfected p23 keratinocyte cell line  vector transfected Uninfected
GSM1422234 NOK cl1 pTURF transfected p24 keratinocyte cell line  vector transfected Uninfected
GSM1422235 NOK cl1 AkataBX-1 cl4 p31 EBV+ keratinocyte cell line  EBV Human gammaherpesvirus 4
GSM1422236 NOK cl1 AkataBX-1 cl4 p32 EBV+ keratinocyte cell line  EBV Human gammaherpesvirus 4
GSM1422237 NOK cl1 AkataBX-1 cl1 p29 EBV- keratinocyte cell line  EBV(-) Uninfected Extra interventions
transiently infected
GSM1422238 NOK cl1 AkataBX-1 cl1 p30 EBV- keratinocyte cell line  EBV(-) Uninfected Extra interventions
transiently infected
GSM1422239 NOK cl1 AkataBX-1 cl3 p29 EBV- keratinocyte cell line  EBV(-) Uninfected Extra interventions
transiently infected
GSM1422240 NOK cl1 AkataBX-1 cl3 p30 EBV- keratinocyte cell line  EBV(-) Uninfected Extra interventions
transiently infected
GSM1422241 NOK cl1 AkataBX-1 cl4 p29 EBV- keratinocyte cell line  EBV(-) Uninfected Extra interventions
transiently infected
GSM1422242 NOK cl1 AkataBX-1 cl4 p30 EBV- keratinocyte cell line  EBV(-) Uninfected Extra interventions
transiently infected