Virus Expression Database
Submitted by Caroline Friedel (Ludwig-Maximilians-Universität München, Germany) on Jul 23 2014
Platform: ngs – Illumina HiSeq 2500 (Homo sapiens)
Pubmed: 25989971, 29579120, 28135292
Summary Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. Newly transcribed RNA was labelled by adding 500µM 4-thiouridine (4sU) to the cell culture media for 1h. Total cellular RNA was isolated using Trizol. Newly transcribed RNA was purified following the protocol described in Raedle et al. JoVE 2013.
| ID | Title | Cell Type | Timepoint | Reported Virus | Virus Species | Exclusion Reason |
|---|---|---|---|---|---|---|
| GSM1444166 | Total RNA - mock Rep 1 | dermal fibroblast  |
mock | Uninfected | ||
| GSM1444167 | Total RNA - 2hpi Rep 1 | dermal fibroblast |
2 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | |
| GSM1444168 | Total RNA - 4hpi Rep 1 | dermal fibroblast |
4 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | |
| GSM1444169 | Total RNA - 6hpi Rep 1 | dermal fibroblast |
6 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | |
| GSM1444170 | Total RNA - 8hpi Rep 1 | dermal fibroblast |
8 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | |
| GSM1444171 | 4sU-RNA - mock Rep 1 | dermal fibroblast |
mock | Uninfected | Extra interventions 4sU-RNA (only newly transcribed) |
|
| GSM1444172 | 4sU-RNA - 0-1 hpi Rep 1 | dermal fibroblast |
1 hr | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444173 | 4sU-RNA - 1-2 hpi Rep 1 | dermal fibroblast |
2 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444174 | 4sU-RNA - 2-3 hpi Rep 1 | dermal fibroblast |
3 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444175 | 4sU-RNA - 3-4 hpi Rep 1 | dermal fibroblast |
4 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444176 | 4sU-RNA - 4-5 hpi Rep 1 | dermal fibroblast |
5 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444177 | 4sU-RNA - 5-6 hpi Rep 1 | dermal fibroblast |
6 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444178 | 4sU-RNA - 6-7 hpi Rep 1 | dermal fibroblast |
7 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444179 | 4sU-RNA - 7-8 hpi Rep 1 | dermal fibroblast |
8 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444180 | Total RNA - mock Rep 2 | dermal fibroblast |
mock | Uninfected | ||
| GSM1444181 | Total RNA - 2hpi Rep 2 | dermal fibroblast |
2 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | |
| GSM1444182 | Total RNA - 4hpi Rep 2 | dermal fibroblast |
4 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | |
| GSM1444183 | Total RNA - 6hpi Rep 2 | dermal fibroblast |
6 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | |
| GSM1444184 | Total RNA - 8hpi Rep 2 | dermal fibroblast |
8 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | |
| GSM1444185 | 4sU-RNA - mock Rep 2 | dermal fibroblast |
mock | Uninfected | Extra interventions 4sU-RNA (only newly transcribed) |
|
| GSM1444186 | 4sU-RNA - 0-1 hpi Rep 2 | dermal fibroblast |
1 hr | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444187 | 4sU-RNA - 1-2 hpi Rep 2 | dermal fibroblast |
2 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444188 | 4sU-RNA - 2-3 hpi Rep 2 | dermal fibroblast |
3 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444189 | 4sU-RNA - 3-4 hpi Rep 2 | dermal fibroblast |
4 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444190 | 4sU-RNA - 4-5 hpi Rep 2 | dermal fibroblast |
5 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444191 | 4sU-RNA - 5-6 hpi Rep 2 | dermal fibroblast |
6 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444192 | 4sU-RNA - 6-7 hpi Rep 2 | dermal fibroblast |
7 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |
| GSM1444193 | 4sU-RNA - 7-8 hpi Rep 2 | dermal fibroblast |
8 hrs | HSV-1 strain 17 | Human alphaherpesvirus 1 | Extra interventions 4sU-RNA (only newly transcribed) |