GSE61141

Phenotypic responses of differentiated asthmatic human airway epithelial cultures to rhinovirus

Submitted by Yongsheng Huang (University of Michigan, USA) on Sep 05 2014

Platform: ngs – Illumina HiSeq 2000 (Homo sapiens)

Pubmed: 25706956

Summary We report the application of RNA sequencing technology for high-throughput profiling of gene expression responses to human rhinovirus infection at 24 hours in air-liquid interface human airway epithelial cell cultures derived from 6 asthmatic and 6 non-asthmatic donors. RNA-seq analysis identified sets of genes associated with asthma specific viral responses. These genes are related to inflammatory pathways, epithelial remodeling and cilium assembly and function, including those described previously (e.g. CCL5, CXCL10 and CX3CL1), and novel ones that were identified for the first time in this study (e.g. CCRL1, CDHR3). We concluded that air liquid interface cultured human airway epithelial cells challenged with live HRV are a useful in vitro model for the study of rhinovirus induced asthma exacerbation, given that our findings are consistent with clinical data sets. Furthermore, our data suggest that abnormal airway epithelial structure and inflammatory signaling are important contributors to viral induced asthma exacerbation.

24 Samples

ID	Title	Cell Type	Timepoint	Reported Virus	Virus Species	Exclusion Reason
GSM1498304	s12A24HRV	tracheobronchial epithelial cell	24 hrs	HRV16	Rhinovirus A	Extra interventions asthmatic
GSM1498305	s14A24HRV	tracheobronchial epithelial cell	24 hrs	HRV16	Rhinovirus A	Extra interventions asthmatic
GSM1498306	s28A24HRV	tracheobronchial epithelial cell	24 hrs	HRV16	Rhinovirus A	Extra interventions asthmatic
GSM1498307	s18A24HRV	tracheobronchial epithelial cell	24 hrs	HRV16	Rhinovirus A	Extra interventions asthmatic
GSM1498308	s15A24HRV	tracheobronchial epithelial cell	24 hrs	HRV16	Rhinovirus A	Extra interventions asthmatic
GSM1498309	s26A24HRV	tracheobronchial epithelial cell	24 hrs	HRV16	Rhinovirus A	Extra interventions asthmatic
GSM1498310	s28A24Veh	tracheobronchial epithelial cell	24 hrs	Vehicle	Uninfected	Extra interventions asthmatic
GSM1498311	s12A24Veh	tracheobronchial epithelial cell	24 hrs	Vehicle	Uninfected	Extra interventions asthmatic
GSM1498312	s26A24Veh	tracheobronchial epithelial cell	24 hrs	Vehicle	Uninfected	Extra interventions asthmatic
GSM1498313	s15A24Veh	tracheobronchial epithelial cell	24 hrs	Vehicle	Uninfected	Extra interventions asthmatic
GSM1498314	s18A24Veh	tracheobronchial epithelial cell	24 hrs	Vehicle	Uninfected	Extra interventions asthmatic
GSM1498315	s14A24Veh	tracheobronchial epithelial cell	24 hrs	Vehicle	Uninfected	Extra interventions asthmatic
GSM1498316	s30N24HRV	tracheobronchial epithelial cell	24 hrs	HRV16	Rhinovirus A
GSM1498317	s23N24HRV	tracheobronchial epithelial cell	24 hrs	HRV16	Rhinovirus A
GSM1498318	s20N24HRV	tracheobronchial epithelial cell	24 hrs	HRV16	Rhinovirus A
GSM1498319	s11N24HRV	tracheobronchial epithelial cell	24 hrs	HRV16	Rhinovirus A
GSM1498320	s22N24HRV	tracheobronchial epithelial cell	24 hrs	HRV16	Rhinovirus A
GSM1498321	s21N24HRV	tracheobronchial epithelial cell	24 hrs	HRV16	Rhinovirus A
GSM1498322	s21N24Veh	tracheobronchial epithelial cell	24 hrs	Vehicle	Uninfected
GSM1498323	s23N24Veh	tracheobronchial epithelial cell	24 hrs	Vehicle	Uninfected
GSM1498324	s20N24Veh	tracheobronchial epithelial cell	24 hrs	Vehicle	Uninfected
GSM1498325	s22N24Veh	tracheobronchial epithelial cell	24 hrs	Vehicle	Uninfected
GSM1498326	s11N24Veh	tracheobronchial epithelial cell	24 hrs	Vehicle	Uninfected
GSM1498327	s30N24Veh	tracheobronchial epithelial cell	24 hrs	Vehicle	Uninfected