GSE71045

Gene expression of CD8+ T cells isolated from human subjects during acute and convalescent phase of EBV infection

Submitted by Thomas C Greenough (University of Massachusetts Medical School, USA) on Jul 17 2015

Platform: microarray – [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]

Pubmed: 26416268

Summary Affymetrix HuGene ST 1.0 microarrays were used to study and compare gene expression in peripheral blood CD8+ T cells of human patients with Acute Infectious Mononucleosis (AIM; acute EBV infection) and during convalescence (CONV; 6-12 months after AIM visit). Blood samples were drawn from ten human patients with AIM and again during their covalescence (CONV). Peripheral blood mononuclear cells were isolated and cryopreserved. Paired AIM and CONV samples were thawed and CD8+ T cells purified with magnetic beads. RNA was isolated and processed for hybridization according to the Affymetrix protocol

20 Samples

ID	Title	Cell Type	Timepoint	Reported Virus	Virus Species
GSM1826035	E1339_AIM	cytotoxic T-lymphocyte	acute infection	EBV	Human gammaherpesvirus 4
GSM1826036	E1339_CONV	cytotoxic T-lymphocyte	convalescence	EBV	Human gammaherpesvirus 4
GSM1826037	E1354_AIM	cytotoxic T-lymphocyte	acute infection	EBV	Human gammaherpesvirus 4
GSM1826038	E1354_CONV	cytotoxic T-lymphocyte	convalescence	EBV	Human gammaherpesvirus 4
GSM1826039	E1369_AIM	cytotoxic T-lymphocyte	acute infection	EBV	Human gammaherpesvirus 4
GSM1826040	E1369_CONV	cytotoxic T-lymphocyte	convalescence	EBV	Human gammaherpesvirus 4
GSM1826041	E1420_AIM	cytotoxic T-lymphocyte	acute infection	EBV	Human gammaherpesvirus 4
GSM1826042	E1420_CONV	cytotoxic T-lymphocyte	convalescence	EBV	Human gammaherpesvirus 4
GSM1826043	E1427_AIM	cytotoxic T-lymphocyte	acute infection	EBV	Human gammaherpesvirus 4
GSM1826044	E1427_CONV	cytotoxic T-lymphocyte	convalescence	EBV	Human gammaherpesvirus 4
GSM1826045	E1435_AIM	cytotoxic T-lymphocyte	acute infection	EBV	Human gammaherpesvirus 4
GSM1826046	E1435_CONV	cytotoxic T-lymphocyte	convalescence	EBV	Human gammaherpesvirus 4
GSM1826047	E1405_AIM	cytotoxic T-lymphocyte	acute infection	EBV	Human gammaherpesvirus 4
GSM1826048	E1405_CONV	cytotoxic T-lymphocyte	convalescence	EBV	Human gammaherpesvirus 4
GSM1826049	E1438_AIM	cytotoxic T-lymphocyte	acute infection	EBV	Human gammaherpesvirus 4
GSM1826050	E1438_CONV	cytotoxic T-lymphocyte	convalescence	EBV	Human gammaherpesvirus 4
GSM1826051	E1376_AIM	cytotoxic T-lymphocyte	acute infection	EBV	Human gammaherpesvirus 4
GSM1826052	E1376_CONV	cytotoxic T-lymphocyte	convalescence	EBV	Human gammaherpesvirus 4
GSM1826053	E1382_AIM	cytotoxic T-lymphocyte	acute infection	EBV	Human gammaherpesvirus 4
GSM1826054	E1382_CONV	cytotoxic T-lymphocyte	convalescence	EBV	Human gammaherpesvirus 4