Virus Expression Database

GSE84897

Genome-wide analysis of DNA hydroxymethylation and methylation during epstein-barr virus infection.

Submitted by Masaki Fukuyo (Chiba University, Japan) on Jul 27 2016

Platform: ngs – Illumina NextSeq 500 (Homo sapiens)

Pubmed: 27829228

Summary Extensive DNA methylation in promoter regions is observed in gastric cancer with Epstein-barr virus (EBV) infection and EBV infection is the cause to induce this extensive hypermethylaiton phenotype in gastric epithelial cells. From transcriptome analysis, we found that TET2, one of the demethylase enzymes, was downregulated by EBV infection in gastric epithelial cell line MKN7. TET2 was overexpressed in a gastric epithelial cell line, GES1, to see its function and the hydroxymethylation, a byproduct of DNA demethylation, acquired genes by TET2 overexpression and methylation acquired genes by EBV infection were significantly overlapped. These suggested that hydroxymethylation by TET2 could function to keep unmethylated status of genes before EBV infection, and TET2 depression could contribute to methylation acquisition of these target genes after EBV infection.

4 Samples

ID Title Cell Type Timepoint Reported Virus Virus Species Exclusion Reason
GSM2253673 MKN7_WT_RNAseq gastric adenocarcinoma cell line  [MKN7 ] none Uninfected
GSM2253674 MKN7_EB#1_RNAseq gastric adenocarcinoma cell line  [MKN7 ] unknown EBV Human gammaherpesvirus 4
GSM2253675 MKN7_EB#2_RNAseq gastric adenocarcinoma cell line  [MKN7 ] unknown EBV Human gammaherpesvirus 4
GSM2253676 MKN7_EB#3_RNAseq gastric adenocarcinoma cell line  [MKN7 ] unknown EBV Human gammaherpesvirus 4