Virus Expression Database

GSE85599

Whole transcriptome profiling reveals major cell types in the cellular immune response against acute and chronic active Epstein-Barr virus infection

Submitted by Jinsheng Yu (Washington University, USA) on Aug 15 2016

Platform: microarray – [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]

Pubmed: 29259291

Summary Epstein–Barr virus (EBV) is a common human pathogen that infects over 95% of the population worldwide. In the present study, the whole transcriptome microarray data were generated from peripheral blood mononuclear cells from Chinese children with acute infectious mononucleosis (AIM) and chronic active EBV infection (CAEBV) that were also compared with a publicly available microarray dataset from a study of American college students with AIM. Our study characterized for the first time a broad spectrum of molecular signatures in AIM and CAEBV. The key findings from the transcriptome profiling were validated with qPCR and flow cytometry assays. The most important finding in our study is the discovery of predominant γδ TCR expression and γδ T cell expansion in AIM. This finding, in combination with the striking up-regulation of CD3, CD8 and CD94, suggests that CD8+ T cells and CD94+ NK cells may play a major role in AIM. Moreover, the unique up-regulation of CD64A/B and its significant correlation with the monocyte marker CD14 was observed in CAEBV and that implies an important role of monocytes in CAEBV. In conclusion, our study reveals major cell types (particularly γδ T cells) in the host cellular immune response against AIM and CAEBV.

17 Samples

ID Title Cell Type Timepoint Reported Virus Virus Species Exclusion Reason
GSM2279022 Acute EBV 15VC1799 peripheral blood mononuclear cell  acute EBV Human gammaherpesvirus 4
GSM2279023 Acute EBV 15VC1803 peripheral blood mononuclear cell  acute EBV Human gammaherpesvirus 4
GSM2279024 Acute EBV 15VC2293 peripheral blood mononuclear cell  acute EBV Human gammaherpesvirus 4
GSM2279025 Chronic EBV 15VC2295 peripheral blood mononuclear cell  chronic EBV Human gammaherpesvirus 4
GSM2279026 Acute EBV 15VC2386 peripheral blood mononuclear cell  acute EBV Human gammaherpesvirus 4
GSM2279027 Chronic EBV 15VC2407 peripheral blood mononuclear cell  chronic EBV Human gammaherpesvirus 4
GSM2279028 Chronic EBV 15VC2414 peripheral blood mononuclear cell  chronic EBV Human gammaherpesvirus 4
GSM2279029 Chronic EBV 15VC2656 peripheral blood mononuclear cell  chronic EBV Human gammaherpesvirus 4
GSM2279030 Chronic EBV 15VC3454 peripheral blood mononuclear cell  chronic EBV Human gammaherpesvirus 4
GSM2279031 Healthy Control C13 peripheral blood mononuclear cell  Control Uninfected
GSM2279032 Healthy Control C15 peripheral blood mononuclear cell  Control Uninfected
GSM2279033 Healthy Control C21 peripheral blood mononuclear cell  Control Uninfected
GSM2279034 Healthy Control C22 peripheral blood mononuclear cell  Control Uninfected
GSM2279035 Healthy Control C23 peripheral blood mononuclear cell  Control Uninfected
GSM2279036 Healthy Control C24 peripheral blood mononuclear cell  Control Uninfected Data failed QC
all_probeset_mad_residual_mean is 0.809375 (poor normalization, above threshold of 0.80)
GSM2279037 Acute EBV ED483 peripheral blood mononuclear cell  acute EBV Human gammaherpesvirus 4
GSM2279038 Acute EBV ED490 peripheral blood mononuclear cell  acute EBV Human gammaherpesvirus 4