GSE87750

Zika virus directly infects human peripheral neurons and induces cell death

Submitted by Peng Jin (Emory University, USA) on Dec 14 2016

Platform: ngs – Illumina HiSeq 3000 (Homo sapiens)

Pubmed: 28758997

Summary Zika virus (ZIKV), a flavivirus transmitted primarily by Aedes mosquitoes, has spread to 59 countries and territories throughout the world. ZIKV-infected patients can have several symptoms and there is scientific consensus that ZIKV is a cause of microcephaly. Most studies are focusing on the central nervous system (CNS), and it is largely unknown whether ZIKV also affects the developing peripheral nervous system. Here, using a human pluripotent stem cell-based model, we demonstrated that Puerto Rican ZIKV strain, PRVABC59, efficiently infects human neural crest cells (hNCCs). In contrast to little infection of CNS neurons, ZIKV readily infects human peripheral neurons (hPNs), leading to cell death. Global gene expression analyses of infected hNCCs and hPNs reveal transcriptional dysregulation, notably of cell-death- and cell-cycle-related pathways. Our results identify hNCCs and hPNs as a direct ZIKV target and provide a framework to investigate the ZIKV pathology on the peripheral neurons.

13 Samples

ID	Title	Cell Type	Timepoint	Reported Virus	Virus Species	Exclusion Reason
GSM2339848	RNAseq-hPN-mock-1	neuron		mock	Uninfected
GSM2339849	RNAseq-hPN-mock-2	neuron		mock	Uninfected
GSM2339850	RNAseq-hPN-ZIKVPR-1	neuron	unknown	ZIKV PRVABC59	Zika virus	Data failed QC pseudoaligned_reads is 772117 (low number of usable reads) pct_pseudoaligned is 15.9% (low fraction of usable reads)
GSM2339851	RNAseq-hPN-ZIKVPR-2	neuron	unknown	ZIKV PRVABC59	Zika virus
GSM2339852	RNAseq-hNCC-mock-1	neural crest cell		mock	Uninfected	Data failed QC pct_pseudoaligned is 17.3% (low fraction of usable reads)
GSM2339853	RNAseq-hNCC-mock-2	neural crest cell		mock	Uninfected
GSM2339854	RNAseq-hNCC-ZIKVPR-1	neural crest cell	unknown	ZIKV PRVABC59	Zika virus	Data failed QC pct_pseudoaligned is 15.1% (low fraction of usable reads)
GSM2339855	RNAseq-hNCC-ZIKVPR-2	neural crest cell	unknown	ZIKV PRVABC59	Zika virus
GSM2429081	RNAseq-hPN-ZIKVPR-3	neuron	unknown	ZIKV PRVABC59	Zika virus
GSM2429082	RNAseq-hPN-ZIKVPR-4	neuron	unknown	ZIKV PRVABC59	Zika virus
GSM2429083	RNAseq-hNCC-mock-2 [HiSeq 3000]	neural crest cell		mock	Uninfected
GSM2429084	RNAseq-hNCC-ZIKVPR-1 [HiSeq 3000]	neural crest cell	unknown	ZIKV PRVABC59	Zika virus	Data failed QC pct_pseudoaligned is 12.4% (low fraction of usable reads)
GSM2429085	RNAseq-hNCC-ZIKVPR-2 [HiSeq 3000]	neural crest cell	unknown	ZIKV PRVABC59	Zika virus