Submitted by Jian Ma (Central South University, China) on Mar 02 2017
Platform: ngs – Illumina Genome Analyzer IIx (Homo sapiens)
Pubmed: 28877527
Summary Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are using transcriptome profiling (RNA-seq) to evaluate the effects of EBV infection or (and) EBV BART6-3p mimics on the global transcriptome of the BJAB cells.
Methods: BJAB cells were transfected with negative control mimics or BART6-3p mimics for 48 h and then infected with EBV virons for 2h. RNAs were extracted by Trizol and sequenced by Solexa high-throughput sequencing service (Oebiotech, Shanghai, China). Data were extracted and normalized according to the manufacturer’s standard protocol.
Results: Log-fold changes of up- or down-regulated mRNAs between the control and experiment group were selected with a significance threshold of p<0.05. There are 408 mRNAs were up-regulated and 263 were down-regulated in “EBV infection” group cells comparing to “Mock” group cells. There are 385 mRNAs were up-regulated and 246 were down-regulated in “EBV infection + Bart6-3p mimics” group cells comparing to “EBV infection + negative control mimics” group cells.
Conclusions: Our study describes the global transciptome changes of BJAB cells induced by EBV infection or (and) EBV 6-3p mimics.
ID | Title | Cell Type | Timepoint | Reported Virus | Virus Species | Exclusion Reason |
---|---|---|---|---|---|---|
GSM2520033 | Mock | B-lymphoma cell line ![]() ![]() |
mock | Uninfected | ||
GSM2520034 | EBV | B-lymphoma cell line ![]() ![]() |
2 hrs | EBV | Human gammaherpesvirus 4 | |
GSM2520035 | EBV+NC | B-lymphoma cell line ![]() ![]() |
2 hrs | EBV | Human gammaherpesvirus 4 | Extra interventions transfected with "negative control" miRNAs |
GSM2520036 | EBV+6-3p | B-lymphoma cell line ![]() ![]() |
2 hrs | EBV | Human gammaherpesvirus 4 | Extra interventions transfected with BART6-3p mimics |